Effects of perfluorooctanoic acid on endoplasmic reticulum stress and lipid metabolism-related genes in human pancreatic cells

被引：1

作者：

Abudayyak, Mahmoud ^{[1
]}

Karaman, Ecem Fatma ^{[2
]}

Guler, Zeynep Rana ^{[1
,3
]}

Ozden, Sibel ^{[1
,4
]}

机构：

[1] Istanbul Univ, Fac Pharm, Dept Pharmaceut Toxicol, Istanbul, Turkiye

[2] Biruni Univ, Fac Pharm, Dept Pharmaceut Toxicol, Istanbul, Turkiye

[3] Istanbul Univ, Inst Grad Studies Hlth Sci, Istanbul, Turkiye

[4] Istanbul Univ, Fac Pharm, Dept Pharmaceut Toxicol, TR-34116 Istanbul, Turkiye

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2023年 / 98卷

关键词：

Endoplasmic reticulum stress; Epigenetics; Metabolism-related genes; Pancreatitis; Perfluorooctanoic acid; PERFLUOROALKYL SUBSTANCES; GLUCOSE-HOMEOSTASIS; OXIDATIVE STRESS; EXPOSURE; EXPRESSION; PFOA; MOUSE; LIVER; METHYLATION; ASSOCIATION;

D O I：

10.1016/j.etap.2023.104083

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Perfluorooctanoic acid (PFOA) is environmentally persistent and has been classified by The International Cancer Research Agency (IARC) as a possible human pancreatic carcinogen. In this study, the epigenetic alteration, the changes in the expression levels of endoplasmic reticulum stress-related and metabolism-related genes, as well as DNA methyltransferase expression were investigated using RT-PCR and ELISA assays. PFOA induced a significant increase in the methylation ratio (5-mC%), impacted DNA methylation maintenance gene expression and decreased lipid metabolism-related genes except for PPAR gamma (>= 13-fold increase). While PFOA induced the expression of ATF4 (>= 5.41-folds), CHOP (>= 5.41-folds) genes, it inhibited the expression of ATF6 (>= 67.2%), GRP78 (>= 64.3%), Elf2 alpha (>= 95.8%), IRE1 (>= 95.5%), and PERK (>= 91.7%) genes. It is thought that epigenetic mechanisms together with disruption in the glucose-lipid metabolism and changes in endoplasmic reticulum stress-related genes may play a key role in PFOA-induced pancreatic toxicity.

引用

页数：10

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