Dendrobium officinale Polysaccharides Inhibit CDCA-Induced Gastric Intestinal Metaplasia through Activating NRF2/HO-1 and Modulating HNF4α/CDX2 Signaling Pathway

被引：0

作者：

Zhao, Yi ^{[1
]}

Huang, Hongxia ^{[1
]}

Wang, Xinnan ^{[1
]}

Hu, Wenxiu ^{[1
]}

Lu, Xuefeng ^{[1
]}

Tang, Suyuan ^{[1
]}

Liu, Hongning ^{[1
]}

Sun, Youzhi ^{[2
]}

机构：

[1] Jiangxi Univ Chinese Med, Res Ctr Differentiat & Dev Basic Theory Tradit Chi, Nanchang, Peoples R China

[2] Jiangxi Univ Chinese Med, Sch Chinese Med, Nanchang, Peoples R China

来源：

JOURNAL OF FOOD BIOCHEMISTRY | 2023年 / 2023卷

基金：

中国国家自然科学基金;

关键词：

PROTECTIVE ROLES; TFF2; EXPRESSION; UP-REGULATION; APOPTOSIS; CHILDREN;

D O I：

10.1155/2023/6668818

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bile reflux (BR) was considered to be an independent risk factor for the development of precancerous gastric lesions and GC. Dendrobium officinale polysaccharides (DOP) show a novel potential in preventing the progress of gastric cancer. However, the specific mechanism of DOP that causes such activities remains a mystery. This study aimed to investigate the effects of DOP on chenodeoxycholic acid (CDCA)-induced gastric intestinal metaplasia and explore the underlying mechanisms. Different concentrations of DOP had no significant damage to normal GSE-1 cells and gastric intestinal metaplasia model cells by CCK-8 assay. After DOP treatment, the mRNA and protein expression of CDX2 (p < 0.01) and HNF4 alpha (p < 0.01) were decreased, and HO-1 (p < 0.05) and TFF2 (p < 0.01) were increased. The NRF2 protein expression was slightly upregulated (p < 0.05), and H-DOP further promoted NRF2 protein expression in the nucleus (p < 0.05). Hence, our findings reveal that DOP could be used as a potential anti-inflammation supplement by activating NRF2/HO-1 and modulating the HNF4 alpha/CDX2 signaling pathway to inhibit the progress of CDCA-induced gastric intestinal metaplasia.

引用

页数：11

共 63 条

[51] Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro [J].

Xing, Shangping ;

Zhang, Xiaofeng ;

Ke, Hannu ;

Lin, Ji ;

Huang, Yuechun ;

Wei, Gang .

CHEMISTRY CENTRAL JOURNAL, 2018, 12

[52] Artemisia ordosica Polysaccharide Alleviated Lipopolysaccharide-induced Oxidative Stress of Broilers via Nrf2/Keap1 and TLR4/NF-κB Pathway [J].

Xing, Y. Y. ;

Zheng, Y. K. ;

Yang, S. ;

Zhang, L. H. ;

Guo, S. W. ;

Shi, L. L. ;

Xu, Y. Q. ;

Jin, X. ;

Yan, S. M. ;

Shi, B. L. .

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2021, 223

[53] Alteration of gastric microbiota and transcriptome in a rat with gastric intestinal metaplasia induced by deoxycholic acid [J].

Xu, Zijing ;

Xiao, Ling ;

Wang, Shuaishuai ;

Cheng, Yuqin ;

Wu, Jianping ;

Meng, Yufen ;

Bao, Kaifan ;

Zhang, Junfeng ;

Cheng, Chun .

FRONTIERS IN MICROBIOLOGY, 2023, 14

[54] In vivo action of trefoil factor 2 (TFF2) to speed gastric repair is independent of cyclooxygenase [J].

Xue, Lin ;

Aihara, Eitaro ;

Podolsky, Daniel K. ;

Wang, Timothy C. ;

Montrose, Marshall H. .

GUT, 2010, 59 (09) :1184-1191

[55]

Yan MQ, 2019, FASEB J, V33

[56] A Systematic Review of the Mechanisms Underlying Treatment of Gastric Precancerous Lesions by Traditional Chinese Medicine [J].

Yang, Liangjun ;

Li, Jiali ;

Hu, Zhipeng ;

Fan, Xiangzhen ;

Cai, Tiantian ;

Zhou, Hengli ;

Pan, Huafeng .

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2020, 2020

[57] Improvement of antioxidant activity and active ingredient of Dendrobium officinale via microbial fermentation [J].

Yu, Gen ;

Xie, QingFen ;

Su, WenFeng ;

Dai, Shuang ;

Deng, XinYi ;

Gu, QuLiang ;

Liu, Shan ;

Yun, JeonYun ;

Xiang, WenHao ;

Xiong, Yang ;

Yang, GuanDong ;

Ren, Yifei ;

Li, He .

FRONTIERS IN MICROBIOLOGY, 2023, 14

[58] Bile acids promote gastric intestinal metaplasia by upregulating CDX2 and MUC2 expression via the FXR/NF-B signalling pathway [J].

Yu, Jun-Hui ;

Zheng, Jian-Bao ;

Qi, Jie ;

Yang, Kui ;

Wu, Yun-Hua ;

Wang, Kai ;

Wang, Chun-Bao ;

Sun, Xue-Jun .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2019, 54 (03) :879-892

[59] Polysaccharides of Dendrobium officinale Kimura & Migo protect gastric mucosal cell against oxidative damage-induced apoptosis in vitro and in vivo [J].

Zeng, Qiang ;

Ko, Chun-Hay ;

Siu, Wing-Sum ;

Li, Long-Fei ;

Han, Xiao-Qiang ;

Yang, Liu ;

Lau, Clara Bik-San ;

Hu, Jiang-Miao ;

Leung, Ping-Chung .

JOURNAL OF ETHNOPHARMACOLOGY, 2017, 208 :214-224

[60] Comparison of the Antioxidant Activities and Polysaccharide Characterization of Fresh and Dry Dendrobium officinale Kimura et Migo [J].

Zhang, Wang ;

Liu, Xinjie ;

Sun, Xun ;

Han, Rongchun ;

Yu, Nianjun ;

Liang, Juan ;

Zhou, An .

MOLECULES, 2022, 27 (19)

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