Contrast Enhanced Ultrasonography of Kidney in Chronic Intermittent Hypoxia Rat Model

被引:1
作者
Lun, Haimei [1 ,2 ]
Liu, Ruochuan [1 ]
Hu, Qiao [2 ]
Liu, Yaoli [1 ]
Wei, Lisi [2 ]
Liu, Xia [3 ]
Wu, Dan [4 ]
Zhu, Shangyong [1 ,5 ]
机构
[1] Guangxi Med Univ, Dept Med Ultrasound, Affiliated Hosp 1, Nanning, Guangxi, Peoples R China
[2] Peoples Hosp Guangxi Zhuang Autonomous Reg, Dept Ultrasound, Nanning, Guangxi, Peoples R China
[3] Peoples Hosp Guangxi Zhuang Autonomous Reg, Dept Pathol, Nanning, Guangxi, Peoples R China
[4] Peoples Hosp Guangxi Zhuang Autonomous Reg, Dept Pediat, Nanning, Guangxi, Peoples R China
[5] Guangxi Med Univ, Dept Med Ultrasound, Affiliated Hosp 1, 6 Shuangyong Rd, Nanning 530021, Guangxi, Peoples R China
关键词
animal experimentation; chronic intermittent hypoxia; contrast-enhanced ultrasound; obstructive sleep apnea; renal perfusion; OBSTRUCTIVE SLEEP-APNEA; ULTRASOUND; PERFUSION; INJURY;
D O I
10.1002/jum.16147
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
ObjectivesThe objective of our study was to assess the ability of contrast-enhanced ultrasound (CEUS) in evaluating renal microperfusion in an animal model. MethodsTwenty Sprague-Dawley rats were subdivided into two groups: the normal and chronic intermittent hypoxia (CIH) groups. In the CIH model, 10 Sprague-Dawley rats were exposed to CIH for 8 weeks to mimic obstructive sleep apnea (OSA). The CEUS parameters of the renal cortex and medulla were obtained and compared between groups. The pathological changes of the kidney tissues were examined by histological staining such as hematoxylin and eosin (H&E) and Masson's trichrome. ResultsCIH caused morphological damage to kidneys. In the cortex, the peak intensity (PI) (P = .009) was significantly lower and time to peak (Ttop) (P = .019) was significantly prolonged in the CIH group compared with the controls. The area under ascending curve (WiAUC) in the medulla and cortex were both significantly lower in the CIH group than those in the control group (P both <.05). CEUS parameters (including PI and WiAUC of the cortex and WiAUC of the medulla) were negatively correlated with serum creatinine (P all <.05). In the medulla, the area under descending curve (WoAUC) was positively correlated with serum creatinine (P = .027), PI was negatively correlated with uric acid (P = .034). ConclusionCEUS parameters (including Ttop, PI, WoAUC, and WiAUC) reflect renal microvascular changes in CIH. CEUS could be a safe and accurate imaging method to assess renal microvascular damage in CIH rats.
引用
收藏
页码:1319 / 1325
页数:7
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