UV-B regulates seasonal greening of albino leaves by modulating CsHY5-inhibiting chlorophyll biosynthesis in Camellia sinensis cv. Huangkui

被引：4

作者：

Liu, Xuyang ^{[1
,2
,3
]}

Cheng, Xin ^{[1
,2
,3
]}

Cao, Jingjie ^{[1
,2
,3
]}

Zhu, Wenfeng ^{[1
,2
,3
]}

Sun, Ying ^{[1
,2
,3
]}

Lin, Ning ^{[1
,2
,3
]}

Wan, Xiaochun ^{[1
,2
,3
]}

Liu, Linlin ^{[1
,2
,3
,4
]}

机构：

[1] State Key Lab Tea Plant Biol & Utilizat, Hefei, Peoples R China

[2] Tea Proc Minist Agr, Key Lab Tea Biol, Beijing, Peoples R China

[3] Anhui Agr Univ, Anhui Prov Lab Tea Plant Biol & Utilizat, West 130 Changjiang Rd, Hefei 230036, Anhui, Peoples R China

[4] Anhui Agr Univ, West 130 Changjiang Rd, Hefei 230036, Anhui, Peoples R China

来源：

PLANT SCIENCE | 2023年 / 328卷

基金：

中国国家自然科学基金;

关键词：

Chlorophyll; CsHY5; UV-B; Albino; Seasonal greening; Camellia sinensis; GENE-EXPRESSION; LIGHT; TRANSCRIPTION; HY5; INTEGRATION; INTERPLAY;

D O I：

10.1016/j.plantsci.2022.111569

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seasonal greening is crucial for albino plants but the underlying regulatory mechanism is unclear, especially concerning light regulation as one of the most important environmental factors for light-sensitive albino tea plants. Here, we report that the UV-B signal regulates the seasonal greening process of albino leaves by modulating CsHY5-inhibiting chlorophyll biosynthesis in Camellia sinensis cv. Huangkui. Reduction of solar UV-B in plantation promoted the seasonal greening of albino 'HK' leaves by inhibiting CsHY5 transcription and activating genes involved in light-harvesting CsLhlb and the chlorophyll biosynthetic pathway (CsCHLH, CsHEMA1, and CsPORA), leading to enrichment of chlorophyll accumulation and recovery of dysfunctional chloroplasts. In contrast, indoor supplementary UV-B exposure reduced chlorophylls by activating CsHY5 but inhibiting chlorophyll biosynthetic genes. In vivo and in vitro molecular analyses showed that CsHY5 can directly bind to the promoters of CsLhlb, CsCHLH, CsHEMA1, and CsPORA. These results indicate that CsHY5 acts as a repressor for the seasonal greening of the albino tea plants in response to the UV-B signal. This is the first study that investigates the regulatory role of the CsHY5-mediated UV-B signal in regulating the seasonal greening of the albino tea plant, which improves our understanding of light regulation in leaf phenotypes of higher plants.

引用

页数：8