Expression,Purification and Anti-tumor Activity of Curcin

Curcin,purified from the seeds of Jatropha curcas,can be used as a cell-killing agent.Understanding the anti-tumor activity of the recombinant protein of curcin is important for its application inclinical medicine.The segment encoding the mature protein of curcin was inserted into Escherichia colistrain M 15,and the recombinant strain was induced to express by isopropyl-β-D-thiogalactopyranoside at aconcentration of 0.5 mM.The recombinant protein was expressed in the form of inclusion bodies andpurified by Ni-NTA affinity chromatography.The target protein was incubated with the tumor cells atdifferent concentrations for different times and the results demonstrated that the target protein could inhibitthe growth of tumor cells (NCL-H446,SGC-7901 and S180) at 5μg/ml.