miR－18a和miR－21在食管癌中的表达及其对食管癌的诊断价值

被引：13

作者：

姚丽华 ^{[1
]}

汪光蓉 ^{[1
]}

蔡燕 ^{[1
]}

马强 ^{[1
]}

王东生 ^{[1
]}

徐磊 ^{[2
]}

郭晓兰 ^{[1
]}

机构：

[1] 川北医学院附属医院检验科川北医学院医学检验系

[2] 川北医学院转化医学研究中心

来源：

中华肿瘤杂志 | 2019年 / 02期

关键词：

食管肿瘤; miR－18a; miR－21; 肿瘤标志物; 诊断;

D O I：

暂无

中图分类号：

R735.1 [食管肿瘤];

学科分类号：

100214 ;

摘要：

目的探讨miR-18a和miR-21在食管癌中的表达及其对食管癌的诊断价值。方法采用逆转录聚合酶链反应检测miR-18a和miR-21在食管癌细胞系ECA109、KYSE150、TE1和正常食管上皮细胞HET-1A, 45例食管癌组织、癌旁组织, 45例食管癌患者和50名正常对照者外周血血清中的表达。采用化学发光免疫分析仪检测食管癌患者和健康对照者外周血血清中食管癌传统肿瘤标志物癌胚抗原(CEA)、鳞癌抗原(SCC)、Cyrfa21-1和组织多肽抗原(TPA)的浓度。评价miR-18a和miR-21与血清中传统肿瘤标志物对食管癌的诊断效能。结果食管癌细胞ECA109、KYSE150和TE1中miR-18a和miR-21的表达水平分别为1.64±0.17、1.62±0.19、1.46±0.12和20.52±1.48、6.73±0.73、1.43±0.19, 高于正常食管上皮细胞(均P<0.01)。食管癌组织中miR-18a和miR-21的表达分别为32.48±28.62和8.67±11.98, 显著高于癌旁组织(均P<0.001)。食管癌患者外周血血清中miR-18a和miR-21表达水平分别为12.66±11.92和9.15±8.14, 显著高于正常对照组(均P<0.001)。受试者工作特征(ROC)曲线分析显示, miR-18a和miR-21诊断食管癌的曲线下面积分别为0.948和0.9135。与传统食管肿瘤标志物比较, 血清miR-18a和miR-21对食管癌诊断的灵敏度更高, 而联合传统食管肿瘤标志物可进一步提高食管癌诊断的灵敏度和准确度, 分别为97.8%和68.4%。结论 miR-18a和miR-21对食管癌具有诊断价值, 可能成为潜在的新型生物标志物。

引用

页码：107 / 111

页数：5

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