Correlation of brain-derived neurotrophic factor to cognitive impairment following traumatic brain injury in rats

BACKGROUND: In vitro and in vivo studies have confirmed that brain-derived neurotrophic factor (BDNF) can promote survival and differentiation of cholinergic,dopaminergic and motor neurons,and axonal regeneration. BDNF has neuroprotective effects on the nervous system. OBJECTIVE: To explore changes in BDNF expression and cognitive function in rats after brain injury. DESIGN,TIME AND SETTING: The neuropathology experiment was performed at the Second Research Room,Department of Neurosurgery,Fujian Medical University (China) from July 2007 to July 2008. MATERIALS: A total of 72 healthy,male,Sprague Dawley,rats were selected for this study. METHODS: Rat models of mild and moderate traumatic brain injury were created by percussion,according to Feeney’s method (n = 24,each group). A bone window was made in rats from the sham operation group (n = 24),but no attack was conducted. MAIN OUTCOME MEASURES: At days 1,2,4 and 7 following injury,BDNF expression in the rat frontal lobe cortex,hippocampus and basal forebrain was examined by immunohistochemistry (streptavidin-biotin-peroxidase complex method). Changes in rat cognitive function were assessed by the walking test,balance-beam test and memory function detection. RESULTS: Cognitive impairment was aggravated at day 2,and recovered to normal at days 3 and 7 in rats from the mild and moderate traumatic brain injury groups. BDNF expression in the rat frontal lobe cortex,hippocampus and basal forebrain was increased at 1 day,decreased at day 2,and then gradually increased in the mild and moderate traumatic brain injury groups. BDNF expression was greater in rats from the moderate traumatic brain injury group than in the sham operation and mild traumatic brain injury groups (P < 0.05). CONCLUSION: BDNF expression in the rat frontal lobe cortex,hippocampus and basal forebrain is correlated to cognitive impairment after traumatic brain injury. BDNF has a protective effect on cognitive function in rats following injury.