Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells

被引：0
作者：
Dan Wu1
机构：
来源：
Neural Regeneration Research | 2012年 / 7卷 / 17期
基金：
中国国家自然科学基金;
关键词：
Bax inhibitor-1; RNA interference; SH-SY5Y; endoplasmic reticulum stress; tunicamycin; apoptosis; neural regeneration;
D O I：
暂无
中图分类号：
R739.4 [神经系肿瘤];
学科分类号：
100214 ;
摘要：
A human SH-SY5Y neuroblastoma cell line with a low level of Bax inhibitor-1 expression was established by lentivirus-mediated RNA interference and fluorescence-activated cell sorting.In control SH-SY5Y cells,tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis;however,after Bax inhibitor-1 gene knockdown,cell survival rates were significantly decreased and the degree of apoptosis was significantly increased following tunicamycin treatment.In addition,chromatin condensation and apparent apoptotic phenomena,such as marginalization and cytoplasmic vesicles,were observed.Our findings indicate that Bax inhibitor-1 can delay apoptosis induced by endoplasmic reticulum stress.
引用
收藏
页码：1331 / 1337
页数：7
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