Characterization of WAP2 gene in Aegilops tauschii and comparison with homoeologous loci in wheat

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Aegilops tauschii; AP2; gene; miniature terminal inverted repeat element(MITE); Q gene; wheat;
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S512.1 [小麦];
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0901 ;
摘要
The Q/q gene,also known as WAP2,is an important gene for wheat domestication and is a member of the AP2(APETALA2) class of transcription factors.In the present study,we first isolated the W~tAP2 allele(where the superscript"t"refers to the speciese source,in this case"tauschii") on chromosome 5D from Aegilops tauschii Coss.,the D-genome donor species of common wheat.We found that W~tAP2 and the AP2 gene from Arabidopsis share a central core of the AP2 polypeptide,a highly basic 10-amino acid domain,and an AASSGF box,although there are many differences in the 37-amino acid serine-rich acidic domain and the remaining regions.In addition, W~tAP2 was highly homologous to the homoeologous loci on 5A and 5B of wheat at both the nucleotide and amino acid level.However,there were some variations that are probably related to gene function.In the first AP2 domain, the amino acids VYL on the 5D and 5A loci were replaced with LLR on 5B.In the 37-amino acid serine-rich acidic domain,W~tAP2 on 5D had an extra amino acid insertion.There was also a variation at the 329 amino acid position, which is thought to be related to the appearance of free-threshing wheat.At this position,the amino acid is isoleucine on 5A for the Q allele and valine for the q allele,whereas the amino acid is leucine on 5D and 5B.Furthermore, a Stowaway miniature terminal inverted repeat element(MITE) insertion was present in the ninth intron of WAP2 on 5B of all common wheats and partial tetraploid Triticum turgidum wheats.These results provide new clues for studies into the evolutionary biology of WAP2 and the origin of common wheat.
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页码:543 / 551
页数:9
相关论文
共 11 条
[1]  
Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A , B , and D genomes of common wheat ( Triticum aestivum )[J] . Gitte Petersen,Ole Seberg,Merete Yde,Kasper Berthelsen.Molecular Phylogenetics and Evolution . 2006 (1)
[2]   The molecular genetics of crop domestication [J].
Doebley, John F. ;
Gaut, Brandon S. ;
Smith, Bruce D. .
CELL, 2006, 127 (07) :1309-1321
[3]   Updating of transposable element annotations from large wheat genomic sequences reveals diverse activities and gene associations [J].
Sabot, F ;
Guyot, R ;
Wicker, T ;
Chantret, N ;
Laubin, B ;
Chalhoub, B ;
Leroy, P ;
Sourdille, P ;
Bernard, M .
MOLECULAR GENETICS AND GENOMICS, 2005, 274 (02) :119-130
[4]  
Rapid changes of microsatellite flanking sequence in the allopolyploidization of new synthesized hexaploid wheat[J] . Lianquan Zhang,Dengcai Liu,Zehong Yan,Xiujin Lan,Youliang Zheng,Yonghong Zhou.Science in China Series C: Life Sciences . 2004 (6)
[5]  
Molecular characterization of HMW glutenin subunit allele 1Bx14 : further insights into the evolution of Glu-B1-1 alleles in wheat and related species[J] . W. Li,Y. Wan,Z. Liu,K. Liu,X. Liu,B. Li,Z. Li,X. Zhang,Y. Dong,D. Wang.Theoretical and Applied Genetics . 2004 (5)
[6]  
Identification and mapping of genetic loci affecting the free-threshing habit and spike compactness in wheat ( Triticum aestivum L.)[J] . C. Jantasuriyarat,M. I. Vales,C. J. W. Watson,O. Riera-Lizarazu.Theoretical and Applied Genetics . 2004 (2)
[7]  
Transposable elements, genes and recombination in a 215-kb contig from wheat chromosome 5Am[J] . Phillip J. SanMiguel,Wusirika Ramakrishna,Jeffrey L. Bennetzen,Carlos S. Busso,Jorge Dubcovsky.Functional & Integrative Genomics . 2002 (1-2)
[8]  
The Q locus of Iranian and European spelt wheat[J] . M. C. Luo,Z. L. Yang,J. Dvo?ák.TAG Theoretical and Applied Genetics . 2000 (3-4)
[9]  
QTL mapping of genes controlling ear emergence time and plant height on chromosome 5A of wheat[J] . K. Kato,H. Miura,S. Sawada.TAG Theoretical and Applied Genetics . 1999 (3-4)
[10]  
Misdivision of univalents in common wheat[J] . E. -R. Sears.Chromosoma . 1950 (1)