MiR-19a promotes epithelial-mesenchymal transition through PI3K/AKT pathway in gastric cancer

被引:0
作者
Wei-Dong Lu [1 ]
Yun Zuo [1 ]
Zhen Xu [1 ]
Min Zhang [1 ]
机构
[1] Department of Oncology, Zhangjiagang First People’s Hospital, Suzhou University
关键词
Gastric cancer; MiR-19a; PI3K-AKT; Epithelialmesenchymal transition;
D O I
暂无
中图分类号
R735.2 [胃肿瘤];
学科分类号
100214 ;
摘要
AIM: To investigate the mechanism by which mi R-19 a is up-regulated in gastric cancer(GC), which plays an oncogenic role.METHODS: In the present study, we investigated the role of mi R-19 a in gastric tissues as well as two GC cell lines. In vivo, we detected the basal expression level of mi R-19 a using real-time reverse transcription-PCR(RTPCR), and the relevance between expression of mi R-19 a and clinicopathological information was analyzed.In vitro, mi R-19 a was ectopically expressed using overexpression and knock-down strategies.RESULTS: Overexpression of mi R-19 a was significantly associated with metastasis of GC and inferior overall prognosis. However, no significant correlation was f o u n d b e t w e e n m i R- 1 9 a e x p r e s s i o n a n d o t h e r characteristics such as age, gender, tobacco, alcohol or tumor size. Cell proliferation, migration and invasion assays showed that overexpression of mi R-19 a promoted the proliferation, migration and invasion, and that overexpression of mi R-19 a promoted the epithelialmesenchymal transition through activating the PI3K/AKT pathway. Blocking the PI3K/AKT pathway could cancel the effect of mi R-19 a.CONCLUSION: All together, our results suggest that mi R-19 a could be used as a promising therapeutic target in the treatment of GC.
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收藏
页码:4564 / 4573
页数:10
相关论文
共 8 条
[1]   miR-34a regulates cisplatin-induce gastric cancer cell death by modulating PI3K/AKT/survivin pathway [J].
Cao, Weiguo ;
Yang, Weiping ;
Fan, Rong ;
Li, Hao ;
Jiang, Jinsong ;
Geng, Mei ;
Jin, Yening ;
Wu, Yunlin .
TUMOR BIOLOGY, 2014, 35 (02) :1287-1295
[2]   MicroRNA-19a/b regulates multidrug resistance in human gastric cancer cells by targeting PTEN [J].
Wang, Fang ;
Li, Ting ;
Zhang, Bin ;
Li, Hong ;
Wu, Qiong ;
Yang, Li ;
Nie, Yongzhan ;
Wu, Kaichun ;
Shi, Yongquan ;
Fan, Daiming .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 434 (03) :688-694
[3]   Down-Regulation of the miRNA-200 Family at the Invasive Front of Colorectal Cancers with Degraded Basement Membrane Indicates EMT Is Involved in Cancer Progression [J].
Paterson, Emily L. ;
Kazenwadel, Jan ;
Bert, Andrew G. ;
Khew-Goodall, Yeesim ;
Ruszkiewicz, Andrew ;
Goodall, Gregory J. .
NEOPLASIA, 2013, 15 (02) :180-U255
[4]   MicroRNAs as promising biomarkers for gastric cancer [J].
Wang, Fang ;
Sun, Guo-Ping ;
Zou, Yan-Feng ;
Hao, Ji-Qing ;
Zhong, Fei ;
Ren, Wen-Jun .
CANCER BIOMARKERS, 2012, 11 (06) :259-267
[5]  
Relation between microRNA expression and progression and prognosis of gastric cancer: a microRNA expression analysis[J] . Tetsuya Ueda,Stefano Volinia,Hiroshi Okumura,Masayoshi Shimizu,Cristian Taccioli,Simona Rossi,Hansjuerg Alder,Chang-gong Liu,Naohide Oue,Wataru Yasui,Kazuhiro Yoshida,Hiroki Sasaki,Sachiyo Nomura,Yasuyuki Seto,Michio Kaminishi,George A Calin,Carlo M Croce. Lancet Oncology . 2010 (2)
[6]   Epithelial-Mesenchymal Transitions in Development and Disease [J].
Thiery, Jean Paul ;
Acloque, Herve ;
Huang, Ruby Y. J. ;
Angela Nieto, M. .
CELL, 2009, 139 (05) :871-890
[7]  
PI3 kinase/Akt signaling mediates epithelial–mesenchymal transition in hypoxic hepatocellular carcinoma cells[J] . Wei Yan,Yu Fu,Dean Tian,Jiazhi Liao,Mei Liu,Bo Wang,Limin Xia,Qian Zhu,Min Luo. Biochemical and Biophysical Research Communications . 2009 (3)
[8]   The inhibition of tumor cell intravasation and subsequent metastasis via regulation of in vivo tumor cell motility by the tetraspanin CD151 [J].
Zijlstra, Andries ;
Lewis, John ;
DeGryse, Bernard ;
Stuhimann, Heidi ;
Quigley, James P. .
CANCER CELL, 2008, 13 (03) :221-234