miR-145通过IGF1R与IRS1逆转胃癌SGC7901/DDP细胞对顺铂的耐药性

被引：7
|
作者：
朱明霞
周鑫
黄泽波
王同杉
朱伟
束永前
刘平
机构：
[1] 南京医科大学第一附属医院肿瘤科
来源：
南京医科大学学报(自然科学版) | 2016年 / 36卷 / 02期
关键词：
miR-145; 顺铂耐药; IGF1R; IRS1;
D O I：
暂无
中图分类号：
R735.2 [胃肿瘤];
学科分类号：
摘要：
目的 :研究miR-145对胃癌耐顺铂细胞株SGC7901/DDP顺铂耐药的影响。方法 :应用QRT-PCR检测miR-145在胃癌组织、细胞中的表达水平;MTT法和细胞克隆形成实验检测细胞活性与增殖能力;荧光素酶实验验证miR-145的靶基因;Western blot、免疫组化和免疫荧光实验检测相关蛋白表达;流式细胞术检测耐药细胞对顺铂诱导凋亡的影响。结果 :miR-145在胃癌组织、各种胃癌细胞株中呈低表达;在胃癌耐顺铂细胞株SGC7901/DDP中,miR-145呈低表达,IGF1R与IRS1呈高表达;上调miR-145增强SGC7901/DDP细胞对顺铂的敏感性;荧光素酶报告实验证实IGF1R与IRS1为miR-145的靶基因;上调miR-145显著降低靶蛋白表达,抑制SGC7901/DDP细胞增殖,促进顺铂诱导的凋亡。结论 :上调miR-145通过靶向IGF1R与IRS1逆转胃癌细胞对顺铂的耐药性。
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页码：144 / 150
页数：7
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