Effects of Nerve Growth Factor on Proliferation and DNA Synthesis of Cultured Human Fetal Retinal Pigment Epithelium Cells

被引：1

作者：

Wensheng Li Jun WenDeyong JiangJianguang Ding Department of Ophthalmology the Second Affiliated Hospital of Kunming Medical College Kunming China Department of Pharmacology Kunming Medical College Kunming China Department of Ophthal ^{[650101
,650031
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机构：

来源：

眼科学报 | 2002年 / 01期

关键词：

pigment epithelium of eye; nerve growth factor; proliferation; DNA synthesis;

D O I：

暂无

中图分类号：

R774.1 [视网膜疾病];

学科分类号：

100212 ;

摘要：

<正>Objective: To investigate the effects of nerve growth factor(NGF)on proliferation and DNA synthesis of cultured human fetal retinal pigment epithelium (RPE) cells in vitro. Methods: Primary culture and subculture of human fetal retinal pigment epithelium cells were established in vitro first. Cultured RPE cellswere treated with NGF by various concentrations 0μg/L, 50μg/L, 100μg/L,200μ,g/L and 300μg/L(final concentration) for 48 hs. After 48 hs, cells proliferation was measured with methyl thiazolyl tetrazolium (MTT) assay method and the amount of DNA was determined by the absorbance at 280 nm of nucleic acid & protein analysis.Results: The A values of 100μg/L, 200μg/L, 300μg/L NGF was(0. 213 7 ± 0. 233), (0.218 8 ±0. 018 1), (0.232 2 ±0.016 4) as compared with(0. 1897 ±0.0152) of A value of 0 μg/L NGF respectively, q value was 3.63,4.40,6.42 and P value was 0.015, 0.000, 0. 000(q-test).The DNA concentrations of 100μg/L, 200μg/L, 300μg/L and 400μg/L NGF was (981. 220 4±123. 5357), (1375.

引用

页码：45 / 48+29 +29

页数：5