Determination of trace mercury in compost extract by inhibition based glucose oxidase biosensor

被引：0

作者：

刘剑潇 ^{[1
]}

徐祥民 ^{[1
,2
]}

汤琳 ^{[2
]}

曾光明 ^{[1
,2
]}

机构：

[1] College of Environmental Science and Engineering, Ocean University of China

[2] College of Environmental Science and Engineering, Hunan University

来源：

TransactionsofNonferrousMetalsSocietyofChina | 2009年 / 19卷 / 01期

关键词：

mercury; inhibition; glucose oxidase; biosensor; compost;

D O I：

暂无

中图分类号：

TP212.3 [生物传感器、医学传感器];

学科分类号：

080202 ;

摘要：

A novel inhibition based biosensor of glucose oxidase(GOx) for environmental mercury detection was developed. An electropolymerized aniline membrane was prepared on a platinum electrode containing ferrocene as electron transfer mediator, on which GOx was cross-linked by glutaraldehyde. The response of the sensor was based on the current reduction in the electrochemical system by inhibition of mercury against GOx electrode. The detection limit of the inhibition-based sensor for mercury is 0.49 μg/L, and the linear response ranges are 0.49-783.21 μg/L and 783.21 μg/L-25.55 mg/L. The GOx membrane can be completely reactivated after inhibition, and remains 70% of the activity in more than one month. The sensor was used for mercury determination in compost extract with good results.

引用

页码：235 / 240

页数：6

共 4 条

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[3]

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[4]

Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase （GOx） and Ferrocene in Poly（pyrrole） Generated from Ethanol/Water Mixtures. Fiorito P.A,Cordoba D.T,Susana I. J. Brazillian Chem. Soc . 2001

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