四氢嘧啶对人皮肤成纤维细胞衰老的影响

被引：2

作者：

李美妮

李永臻

汪明香

缪增强

双杰

机构：

[1] 青海大学医学院基础医学研究中心

来源：

中国皮肤性病学杂志 | 2023年 / 37卷 / 06期

关键词：

四氢嘧啶; 成纤维细胞; 细胞衰老; 活性氧; 细胞凋亡;

D O I：

10.13735/j.cjdv.1001-7089.202209096

中图分类号：

R751 [皮肤病学];

学科分类号：

100206 ;

摘要：

目的探究四氢嘧啶(ectoine)对人皮肤成纤维细胞(HSFs)是否具有减缓衰老的功效及其具体机制。方法采用不同浓度四氢嘧啶作用于HSFs 24 h, MTT法检测细胞活性，β-半乳糖苷酶(SA-β-gal)染色观察细胞衰老情况，流式细胞术检测细胞调亡和活性氧(ROS)的水平，qPCR检测p16、p21、p53 mRNA的表达水平。结果 MTT结果显示，与对照组相比，各四氢嘧啶处理组对细胞无毒性作用，随着四氢嘧啶浓度增加，HSFs细胞凋亡率、ROS水平以及p16、p21、p53 mRNA表达水平均显著降低(P<0.05)。结论四氢嘧啶可通过降低细胞凋亡率、抑制ROS活性和减少p16、p21、p53 mRNA的表达来减缓HSFs衰老，这将为今后开发以四氢嘧啶作为主要成分针对HSFs的抗衰老产品提供一定的理论依据。

引用

页码：639 / 644

页数：6

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