Effect of captopril or verapamil on intracellular sodium in cultured vascular smooth muscle cells

被引:0
作者
QI JIAN HUA
LU ZHANG
JUN WANG
MIN LU
XIN MING WANG
ZHENG JUN JINShanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai ChinaDepartment of Pharmacology Shanghai Second Medical University Shanghai China [200031 ,200025 ]
机构
关键词
Cultured aortic smooth muscle cells; angiotensin; Ⅱ; captopril; verapamil; sodiumbinding benzofuran isophthalate;
D O I
暂无
中图分类号
Q27 [细胞生物物理学];
学科分类号
071009 ; 090102 ;
摘要
The effects of captopril (Cap) and verapamil (Ver)alone and in combination on intracellular Na+ concentration ([Na+]i) in cultured aortic smooth muscle cells (ASMC) of rabbits was evaluated by a direct measurement of [Na+]i with fluorescent dye sodium-binding benzofuran isophthalate (SBFI) combined with digital image. [Na+]i in resting cells was found to be 11.9 ± 0. 7 mmol/L. Angiotensin II (Ang-II, 0.1-10μmol/L) induced an increase of [Na+]i in concentration-dependent manner. Ver (0.1-10μmol/L) inhibited Ang-II (1 μmol/L)-induced increase in [Na+]i, while Cap enhanced Ang-II-induced increase in [Na+]i at 10μmol/L but not at 0.1-1μmol/L. Ver (0.1-1μmol/L)abolished enhancement of Ang-II-induced increase in [Na+]i by Cap. Thus, the inhibition of Capenhanced [Na+]i by Ver may provide a new hypothesis for the underlying molecular mechanism of synergistic effect of the combination of Ca2+ antagonists and angiotensinconverting enzyme inhibitors in controlling blood pressure.
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页码:47 / 53
页数:7
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