MEASUREMENT AND CORRELATION OF PARTITION COEFFICIENTS OF UROKINASE IN AQUEOUS TWO-PHASE PEG-POTASSIUM PHOSPHATE SYSTEM

被引：0
|
作者：
朱自强
李勉
梅乐和
韩兆熊
机构：
来源：
Chinese Journal of Chemical Engineering | 1996年 / 01期
基金：
中国国家自然科学基金;
关键词：
partition coefficients; urokinase; aqueous two phase system; thermodynamic model;
D O I：
暂无
中图分类号：
TQ016 [化工实验与研究];
学科分类号：
0703 ;
摘要：
Partition coefficients of Urokinase(UK)were measured in aqueous two-phase systems con-taining polyethylene glycol and potassium phosphate at 273.2K.Based on the Diamond-Hsu model,a modified expression was obtained for the correlation of enzyme partitioning in theabove-mentioned systems.Utilizing a modified form,the partitioning data of UK and heteroproteinwere correlated.The results show that the modified model is simple gives good precision.and will fa-cilitate engineering scale-up of aqueous two-phase systems for certain proteins purification.
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页码：22 / 30
页数：9
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