Statistical optimization and anticancer activity of a red pigment isolated from Streptomyces sp. PM4

被引：1

作者：

Valliappan Karuppiah ^{[1
,2
]}

Chandramohan Aarthi ^{[1
]}

Kannan Sivakumar ^{[1
]}

Lakshmanan Kannan ^{[1
]}

机构：

[1] CAS in Marine Biology,Faculty of Marine Sciences,Annamalai University,Parangipettai 608502,Tamil Nadu,India

[2] Marine Biotechnology Laboratory,State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology,Shanghai Jiao TongUniversity,Shanghai 200240,P.R. China

来源：

Asian Pacific Journal of Tropical Biomedicine | 2013年 / 08期

关键词：

Streptomyces; Pigment; Response surface methodology; Anticancer activity;

D O I：

暂无

中图分类号：

Q936 [微生物生物化学];

学科分类号：

071010 ;

摘要：

Objective:To enhance the pigment production byStreptomycessp.PM4 for evaluating its anticancer activity.Methods:Response surface methodology was employed to enhance the production of red pigment fromStreptomycessp.PM4.Optimized pigment was purified and evaluated for the anticancer activity against HT1080,Hep2,HeLa and MCF7 cell lines by MTT assay.Results:Based on the response surface methodology,it could be concluded that maltose (4.06 g),peptone (7.34 g),yeast extract(4.34 g)and tyrosine(2.89 g)were required for the maximum production of pigment(1.68 g/L)by theStreptomycessp.PM4.Optimization of the medium with the above tested features increased the pigment yield by 4.6 fold.Pigment showed the potential anticancer activity against HT1080,HEp-2,HeLa and MCF-7cell lines with the IC50value of 18.5,15.3,9.6 and 8.5 respectively.Conclusions:The study revealed that the maximum amount of pigment could be produced to treat cancer.

引用

页码：650 / 656

页数：7

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