LMM5.1 and LMM5.4,two eukaryotic translation elongation factor 1Alike gene family members,negatively affect cell death and disease resistance in rice

被引：7
作者：
Jiying Zhao [1 ,2 ]
Pengcheng Liu [1 ]
Chunrong Li [1 ]
Yanyan Wang [1 ]
Lequn Guo [1 ]
Guanghuai Jiang [1 ]
Wenxue Zhai [1 ]
机构：
[1] Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
[2] Kaifeng Institute for Food and Drug Control
来源：
Journal of Genetics and Genomics | 2017年 / 44卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Lesion mimic; eEF1A-like gene; Cell death; Disease resistance; Rice;
D O I：
暂无
中图分类号：
Q943.2 [植物基因工程];
学科分类号：
071007 ; 090102 ;
摘要：
Lesion mimic mutant(LMM) genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5,which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae(Xoo) by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5.1 and lmm5.4, which were isolated with a map-based cloning strategy. Remarkably, LMM5.1 and LMM5.4 share a 97.4% amino acid sequence identity, and they each encode a eukaryotic translation elongation factor 1A(e EF1A)-like protein. Besides, LMM5.1 and LMM5.4 were expressed in a tissue-specific and an indicaspecific manner, respectively. In addition, high-throughput m RNA sequencing analysis confirmed that the basal immunity was constitutively activated in the lmm5 mutant. Taken together, these results suggest that the homologous e EF1A-like genes, LMM5.1 and LMM5.4, negatively affect cell death and disease resistance in rice.
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页码：107 / 118
页数：12
相关论文
共 33 条
  • [1] Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR[J]. Fen Lu,Huiqin Wang,Shanzhi Wang,Wendi Jiang,Changlin Shan,Bin Li,Jun Yang,Shiyong Zhang,Wenxian Sun.Journal of Integrative Plant Biology. 2015(07)
  • [2] RLINl,encoding a putative coproporphyrinogen Ⅲ oxidase,is involved in lesion initiation in rice
    Changhui Sun~(a
    [J]. 遗传学报, 2011, 38 (01) : 29 - 37
  • [3] A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice
    Tamiru, Muluneh
    Takagi, Hiroki
    Abe, Akira
    Yokota, Takao
    Kanzaki, Hiroyuki
    Okamoto, Haruko
    Saitoh, Hiromasa
    Takahashi, Hideyuki
    Fujisaki, Koki
    Oikawa, Kaori
    Uemura, Aiko
    Natsume, Satoshi
    Jikumaru, Yusuke
    Matsuura, Hideyuki
    Umemura, Kenji
    Terry, Matthew J.
    Terauchi, Ryohei
    [J]. NEW PHYTOLOGIST, 2016, 210 (04) : 1282 - 1297
  • [4] Characterization and fine mapping of a light-dependent leaf lesion mimic mutant 1 in rice[J] . Jing Wang,Bangquan Ye,Junjie Yin,Can Yuan,Xiaogang Zhou,Weitao Li,Min He,Jichun Wang,Weilan Chen,Peng Qin,Bintian Ma,Yuping Wang,Shigui Li,Xuewei Chen.Plant Physiology and Biochemistry . 2015
  • [5] Crystallization and preliminary X‐ray analysis of the C‐terminal fragment of Ski7 from Saccharomyces cerevisiae[J] . Ji‐Young Lee,Si Hoon Park,Byung‐Cheon Jeong,Hyun Kyu Song.Acta Crystallographica Section F . 2014 (9)
  • [6] Identification and characterization of the interaction between viroplasm-associated proteins from two different plant-infecting reoviruses and eEF-1A of rice
    Zhang Songbai
    Du Zhenguo
    Yang Liang
    Yuan Zhengjie
    Wu Kangcheng
    Li Guangpu
    Wu Zujian
    Xie Lianhui
    [J]. ARCHIVES OF VIROLOGY, 2013, 158 (10) : 2031 - 2039
  • [7] Mechanism of the initiation of mRNA decay: role of eRF3 family G proteins[J] . Shin‐ichi Hoshino.WIREs RNA . 2012 (6)
  • [8] SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice[J] . Xifeng Chen,Liang Hao,Jianwei Pan,Xixi Zheng,Guanghuai Jiang,Yang Jin,Zhimin Gu,Qian Qian,Wenxue Zhai,Bojun Ma.Molecular Breeding . 2012 (2)
  • [9] Nitric Oxide and Protein S-Nitrosylation Are Integral to Hydrogen Peroxide-Induced Leaf Cell Death in Rice1[W][OA][J] . Lin,Aihong,Wang,Yiqin,Tang,Jiuyou,Xue,Peng,Li,Chunlai,Liu,Linchuan,Hu,Bin,Yang,Fuquan,Loake,Gary J,Chu,Chengcai.EN . 2012 (1)
  • [10] A Gain-of-Function Mutation in the Arabidopsis Disease Resistance Gene RPP4 Confers Sensitivity to Low Temperature1[W][OA][J] . Huang,Xiaozhen,Li,Jianyong,Bao,Fei,Zhang,Xiaoyan,Yang,Shuhua.Plant Physiology . 2010 (2)
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