Changes of cytosolic [Ca2+]i in neutrophils in pancreatic microcirculation of rats with caerulein-induced acute pancreatitis under fluid shear stress

AIM:To investigate the fluid shear stress induced changesof[Ca2+]i in neutrophils in pancreatic microcirculation ofexperimental acute pancreatitis(AP).METHODS:Wistar rats(n=36)were randomized into threegroups.A model of AP was established by subcutaneousinjection of caerulein.Low-shear 30 viscometer was usedto provide steady fluid shear stress on separated neutrophils.The mean fluorescent intensity tested by flow cytometrywas used as the indication of[Ca2+]i quantity.RESULTS:Under steady shear,cytosolic[Ca2+]i showedbiphasic changes.The shear rate changed from low to high,[Ca2+]i in different groups decreased slightly and then increasedgradually to a high level(P<0.05).A close correlation wasobserved between the cytosolic[Ca2+]i level and the alterationof fluid shear stress in regional microcirculation of AP.CONCLUSION:The increase of[Ca2+]i is highly related tothe activation of neutrophils,which contributes to neutrophiladhesion to endothelium in the early phase of AP.Theeffect of fluid shear stress on[Ca2+]i may play a crucialrole in pancreatic microcirculatory failure of AP.Zhou ZG,Chen YQ,Liu XB,Hu WM,Tian BL,Chen HQ.Changes of cytosolic[Ca2+]i in neutrophils in pancreaticmicrocirculation of rats with caerulein-induced acute pancreatitisunder fluid shear stress.World J Gastroenterol 2004;10(21):3185-3187http://www.wjgnet.com/1007-9327/10/3185.asp