Hesperidin as a preventive resistance agent in MCF-7 breast cancer cells line resistance to doxorubicin

Objective:To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells（MCF-7/Dox）in cytotoxicity apoptosis and P-glycoprotein（Pgp）expression in combination with doxorubicin.Methods:The cytotoxic properties.50%inhibition concentration(IC;)and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin（MCF-7/Dox）cells were determined using MTT assay.Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange.Immunocytochemistry assay was performed to determine the level and localization of Pgp.Results:Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC;value of 11μmol/L.Thus,combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect（CI>1.0）.Hesperidin did not increase the apoptotic induction,but decreased the Pgp expressions level when combined with doxorubicin in low concentration.Conclusions:Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC;of 11μmol/L.Hesperidin did not increased the apoptotic induction combined with doxorubicin.Cochemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression.