Relationship between Calpain-10 Gene Polymorphism and Insulin Resistance Phenotypes in Chinese

被引:1
作者
郑涓
陈璐璐
黎慧清
机构
[1] Tongji Medical College
[2] China
[3] Wuhan 430022
[4] Department of Endocrinology
[5] Huazhong University of Science and Technology
[6] Union Hospital
关键词
Calpain-10; UCSNP-43; UCSNP-44; insulin resistance;
D O I
暂无
中图分类号
R346 [];
学科分类号
1001 ;
摘要
In order to determine whether the variations in the calpain-10 gene constitutes risk of type 2 diabetes (T2DM) in Chinese, the frequency of UCSNP-43, 44 in 268 adults newly diagnosed with T2DM (according to the 1999 ADA criteria) and 153 non-diabetic control subjects was investigated. For all subjects, the height, weight, waist-to-hip ratio (W/H) and blood pressure, as well as following parameters were measured: (1) 75-g oral glucose tolerance test with insulin, C-peptide, HbA1c and blood lipid profiles; (2) Genomic DNA extracted from peripheral blood lymphocytes was genotyped for UCSNP-43 (calpain-10-g.4852 G/A) and UCSNP-44 (calpain-10-g.4841 T/C) by sequencing a polymerase chain reaction (PCR)-amplified fragment. PCR product was selected by single strand conformation polymorphism (SSCP) and then sequenced. The results showed that there was significant difference between T2DM group and normal control group in allele frequencies, haplotype frequencies, or haplotype combinations of UCSNP-43 and -44 either. But in newly diagnosed T2DM group, it was found that the individuals with the genotype UCSNP-44 T/C+C/C had significantly increased fasting and post-challenge insulin levels (FIns and P2hIns), consistent with reduced insulin sensitivity. In the BMI>25 subgroup, the differences were even more significant. It was demonstrated that the Calpain-10 gene polymorphism UCSNP-44 was associated with insulin sensitivity and FIns and P2hIns in newly diagnosed T2DM, although Calpain-10 doesn’t appear as a major diabetes susceptible gene in this population.
引用
收藏
页码:452 / 455
页数:4
相关论文
共 11 条
  • [1] A calpain-10 gene polymorphism is associated with reduced muscle mRNA levels and insulin resistance. Baier L J,Permana P A,Yang X et al. The Journal of Clinical Investigation . 2000
  • [2] Type 2 diabetes and three calpain-10 gene polymorphisms in Samoans: no evidence of association. Tsai H J,Guangyun S,Daniel E et al. The American Journal of Human Genetics . 2001
  • [3] Comment: CAPN10 alleles are associated with polycystic ovary syndrome. Gonzalez A,Abril E,Roca A et al. The Journal of Clinical Endocrinology . 2002
  • [4] Diabetes[P]. 英国专利:GB201117172D0,2011-11-16
  • [5] Diabetes[P]. 英国专利:GB201117172D0,2011-11-16
  • [6] A genome-wide search for human non-insulin dependent(type 2) diabetes genes reveals a major susceptibility locus in chromosome 2. Hanis C L,Boerwinkle E,Chakraborty R et al. Nature Genetics . 1996
  • [7] Homeostasis model assessment closely mirrors the glucose clamp technique in the assessment of insulin sensitivity. Enzo B,Giovanni T,Maria A et al. Diabetes Care . 2000
  • [8] Insulin sensitivity of glucose disposal and lipolysis: no influence of common genetic variants in IRS-1 and CAPN10. Stumvoll M,Wahl HG,Machicao F et al. Diabetologia . 2002
  • [9] Diabetes[P]. 英国专利:GB201117172D0,2011-11-16
  • [10] Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus. Horikawa Y,Oda N,Cox N J et al. Nature Genetics . 2000