Detection and Comparison of Pathogen of Virus Disease in Pumpkin by RT-PCR and IC-PCR

被引：0
作者：
YANG Guohui ZHANG Zhongkai CUI Chongshi Heilongjiang Academy of Agricultural Science Harbin Heilongjiang PRC College of Horticulture Northeast Agricultural University Harbin Heilongjiang PRC Agricultural Biotechnological Key Laboratory of Yunnan Province Kunming Yunnan PRC [1 ,2 ,3 ,2 ,1 ,150086 ,2 ,150030 ,3 ,650223 ]
机构：
来源：
JournalofNortheastAgriculturalUniversity(EnglishEdition) | 2006年 / 02期
关键词：
pumpkin; virus disease; RT-PCR; IC-PCR;
D O I：
暂无
中图分类号：
S436.42 [瓜类病虫害];
学科分类号：
090401 ; 090402 ;
摘要：
Two kinds of methods RT-PCR and IC-PCR were used to detect pathogen of virus disease of pumpkin and the sensitivity of the two methods was compared. The results showed that PRSV-W and CMV were detected in diseased samples gathered in Yunnan Province, while WMV and CMV were detected in diseased samples gathered in Heilongjiang Province. The sensitivity of RT-PCR is higher than that of IC-PCR, but the effect of IC-PCR in the specialization of bonding reaction and requisition for experiment material is better than that of RT-PCR.
引用
收藏
页码：129 / 132
页数：4
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