Detection and Comparison of Pathogen of Virus Disease in Pumpkin by RT-PCR and IC-PCR

被引：0
作者：
YANG Guohui ZHANG Zhongkai CUI Chongshi Heilongjiang Academy of Agricultural Science Harbin Heilongjiang PRC College of Horticulture Northeast Agricultural University Harbin Heilongjiang PRC Agricultural Biotechnological Key Laboratory of Yunnan Province Kunming Yunnan PRC [1 ,2 ,3 ,2 ,1 ,150086 ,2 ,150030 ,3 ,650223 ]
机构：
来源：
JournalofNortheastAgriculturalUniversity(EnglishEdition) | 2006年 / 02期
关键词：
pumpkin; virus disease; RT-PCR; IC-PCR;
D O I：
暂无
中图分类号：
S436.42 [瓜类病虫害];
学科分类号：
090401 ; 090402 ;
摘要：
Two kinds of methods RT-PCR and IC-PCR were used to detect pathogen of virus disease of pumpkin and the sensitivity of the two methods was compared. The results showed that PRSV-W and CMV were detected in diseased samples gathered in Yunnan Province, while WMV and CMV were detected in diseased samples gathered in Heilongjiang Province. The sensitivity of RT-PCR is higher than that of IC-PCR, but the effect of IC-PCR in the specialization of bonding reaction and requisition for experiment material is better than that of RT-PCR.
引用
收藏
页码：129 / 132
页数：4
相关论文
共 50 条
[21]   Use of one step rt-pcr for detection of Asparagus virus 1 [J].
Tomassoli, L. ;
Zaccaria, A. ;
Tiberini, A. .
JOURNAL OF PLANT PATHOLOGY, 2007, 89 (03) :413-415
[22]   The Adaptation of Silica Capture RT-PCR for the Detection of Potato Virus Y [J].
Bogumiła Zacharzewska ;
Agnieszka Przewodowska ;
Krzysztof Treder .
American Journal of Potato Research, 2014, 91 :525-531
[23]   Rabies virus detection by RT-PCR in decomposed naturally infected brains [J].
David, D ;
Yakobson, B ;
Rotenberg, D ;
Dveres, N ;
Davidson, I ;
Stram, Y .
VETERINARY MICROBIOLOGY, 2002, 87 (02) :111-118
[24]   The Adaptation of Silica Capture RT-PCR for the Detection of Potato Virus Y [J].
Zacharzewska, Bogumia ;
Przewodowska, Agnieszka ;
Treder, Krzysztof .
AMERICAN JOURNAL OF POTATO RESEARCH, 2014, 91 (05) :525-531
[25]   Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR [J].
Goto, Yusuke ;
Yaegashi, Gakuji ;
Kumagai, Yoshihiro ;
Ogasawara, Fusae ;
Goto, Makiko ;
Mase, Masaji .
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2019, 81 (01) :103-106
[26]   Development of a RT-PCR for the Detection of European brown hare syndrome virus [J].
Yang Zexiao ;
Li Lirui ;
Wang Yin ;
Yao Xueping ;
Han Xueqing ;
Yang Zengqi ;
Zhou Bo ;
Wang Kaiyu ;
Zhang Yanming .
ADVANCED MATERIALS AND INFORMATION TECHNOLOGY PROCESSING, PTS 1-3, 2011, 271-273 :410-+
[27]   Direct and rapid detection of porcine epidemic diarrhea virus by RT-PCR [J].
Ishikawa, K ;
Sekiguchi, H ;
Ogino, T ;
Suzuki, S .
JOURNAL OF VIROLOGICAL METHODS, 1997, 69 (1-2) :191-195
[28]   Comparison of the conventional multiplex RT-PCR, real time RT-PCR and Luminex xTAG® RVP fast assay for the detection of respiratory viruses [J].
Choudhary, Manohar L. ;
Anand, Siddharth P. ;
Tikhe, Shamal A. ;
Walimbe, Atul M. ;
Potdar, Varsha A. ;
Chadha, Mandeep S. ;
Mishra, Akhilesh C. .
JOURNAL OF MEDICAL VIROLOGY, 2016, 88 (01) :51-57
[29]   Standardization of RT-PCR Protocol for Detection and Typing of Emerging Food and Mouth Disease Virus in Lebanon [J].
Barbour, Elie K. ;
Ahmadieh, Diana M. ;
Shaib, Houssam A. ;
Harakeh, Steve ;
Kumosani, Taha .
International Journal of Applied Research in Veterinary Medicine, 2014, 12 (03) :201-209
[30]   RT-PCR based diagnosis of infectious bursal disease virus in Pakistan [J].
Zahoor, M. A. ;
Hussain, I. ;
Mansoor, M. K. ;
Masood, S. ;
Khan, Q. M. .
JOURNAL OF DAIRY SCIENCE, 2004, 87 :109-110
12345