Detection and Comparison of Pathogen of Virus Disease in Pumpkin by RT-PCR and IC-PCR

被引：0
|
作者：
YANG Guohui1
2. College of Horticulture
3. Agricultural Biotechnological Key Laboratory of Yunnan Province
机构：
来源：
Journal of Northeast Agricultural University(English Edition) | 2006年 / 02期
关键词：
pumpkin; virus disease; RT-PCR; IC-PCR;
D O I：
暂无
中图分类号：
S436.42 [瓜类病虫害];
学科分类号：
090401 ; 090402 ;
摘要：
Two kinds of methods RT-PCR and IC-PCR were used to detect pathogen of virus disease of pumpkin and the sensitivity of the two methods was compared. The results showed that PRSV-W and CMV were detected in diseased samples gathered in Yunnan Province, while WMV and CMV were detected in diseased samples gathered in Heilongjiang Province. The sensitivity of RT-PCR is higher than that of IC-PCR, but the effect of IC-PCR in the specialization of bonding reaction and requisition for experiment material is better than that of RT-PCR.
引用
收藏
页码：129 / 132
页数：4
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