Arpin contributes to bacterial translocation and development of severe acute pancreatitis

被引:0
作者
Wen-Sheng Deng [1 ]
Jian Zhang [2 ]
Hui Ju [3 ]
Hong-Mei Zheng [1 ]
Jiang Wang [1 ]
Su Wang [2 ]
Dian-Liang Zhang [1 ]
机构
[1] Center of Colon and Rectum,Qingdao Municipal Hospital,Qingdao University
[2] Department of General Surgery,The Affiliated Hospital of Qingdao University
[3] Department of Gastroenterology,The Affiliated Hospital of Qingdao University
关键词
Severe acute pancreatitis; Arpin; Tight junction proteins; Bacterial translocation; Epithelium; Intestinal epithelial barrier;
D O I
暂无
中图分类号
R576 [胰腺疾病];
学科分类号
1002 ; 100201 ;
摘要
AIM: To assess the impact of Arpin protein and tight junction(TJ) proteins in the intestinal mucosa onbacterial translocation in patients with severe acute pancreatitis(SAP).METHODS: Fifty SAP patients were identified as study objects and then classified into two groups according to the presence of bacterial translocation(BT) in the blood [i.e., BT(+) and BT(-)].Twenty healthy individuals were included in the control group.BT was analyzed by polymerase chain reaction, colonic mucosal tissue was obtained by endoscopy and the expression of TJ proteins and Arpin protein was determined using immunofluorescence and western blotting.RESULTS: Bacterial DNA was detected in the peripheral blood of 62.0% of patients(31/50) with SAP.The expression of TJ proteins in SAP patients was lower than that in healthy controls.In contrast, Arpin protein expression in SAP patients was higher than in healthy controls(0.38 ± 0.19 vs 0.28 ± 0.16, P < 0.05).Among SAP patients, those positive for BT showed a higher level of claudin-2 expression(0.64 ± 0.27 vs 0.32 ± 0.21, P < 0.05) and a lower level of occludin(OC)(0.61 ± 0.28 vs 0.73 ± 0.32, P < 0.05) and zonula occludens-1(0.42 ± 0.26 vs 0.58 ± 0.17, P = 0.038) expression in comparison with BT(-) patients.Moreover, the level of Arpin expression in BT(+) patients was higher than in BT(-) patients(0.61 ± 0.28 vs 0.31 ± 0.24, P < 0.05).CONCLUSION: Arpin protein affects the expression of tight junction proteins and may have an impact on BT.These results contribute to a better understanding of the factors involved in bacterial translocation during acute pancreatitis.
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页码:4293 / 4301
页数:9
相关论文
共 12 条
[1]  
Effect of honey on bacterial translocation and intestinal morphology in obstructive jaundice[J]. Cem Gencay,Kemal Kismet,Bulent Kilicoglu,Serap Erel,Mehmet Ali Akkus,Sibel Serin Kilicoglu,Sabahattin Muratoglu,Asli Elif Sunay,Esra Erdemli.World Journal of Gastroenterology. 2008(21)
[2]   Actin-related protein2/3 complex regulates tight junctions and terminal differentiation to promote epidermal barrier formation [J].
Zhou, Kang ;
Muroyama, Andrew ;
Underwood, Julie ;
Leylek, Rebecca ;
Ray, Samriddha ;
Soderling, Scott H. ;
Lechler, Terry .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (40) :E3820-E3829
[3]   Tight junctions in human pancreatic duct epithelial cells [J].
Kojima, Takashi ;
Yamaguchi, Hiroshi ;
Ito, Tatsuya ;
Kyuno, Daisuke ;
Kono, Tsuyoshi ;
Konno, Takumi ;
Sawada, Norimasa .
TISSUE BARRIERS, 2013, 1 (04)
[4]  
Bacteremia in Patients With Acute Pancreatitis as Revealed by 16S Ribosomal RNA Gene-Based Techniques*[J] . Qiurong Li,Chenyang Wang,Chun Tang,Qin He,Ning Li,Jieshou Li.Critical Care Medicine . 2013 (8)
[5]  
Acute spontaneous spinal epidural hematoma: an important differential diagnosis in patients under clopidogrel therapy[J] . Ruben Alejandro Morales Ciancio,Olivier Drain,Ludovic Rillardon,Pierre Guigui.The Spine Journal . 2008 (3)
[6]   X-ray scattering study of activated Arp2/3 complex with bound actin-WCA [J].
Boczkowska, Malgorzata ;
Rebowski, Grzegorz ;
Petoukhov, Maxim V. ;
Hayes, David B. ;
Svergun, Dmitri I. ;
Dominguez, Roberto .
STRUCTURE, 2008, 16 (05) :695-704
[7]   Cell-cell contacts prevent anoikis in primary human colonic epithelial cells [J].
Hofmann, Claudia ;
Obermeier, Florian ;
Artinger, Monika ;
Hausmann, Martin ;
Falk, Werner ;
Schoelmerich, Juergen ;
Rogler, Gerhard ;
Grossmann, Johannes .
GASTROENTEROLOGY, 2007, 132 (02) :587-600
[8]   Management of the critically ill patient with severe acute pancreatitis [J].
Nathens, AB ;
Curtis, JR ;
Beale, RJ ;
Cook, DJ ;
Moreno, RP ;
Romand, JA ;
Skerreft, SJ ;
Stapleton, RD ;
Ware, LB ;
Waldmann, CS .
CRITICAL CARE MEDICINE, 2004, 32 (12) :2524-2536
[9]  
Detection and identification of bacterial DNA in patients with cirrhosis and culture-negative, nonneutrocytic ascites[J] . Hepatology . 2002 (1)
[10]   Acute pancreatitis and bacterial translocation [J].
Cicalese, L ;
Sahai, A ;
Sileri, P ;
Rastellini, C ;
Subbotin, V ;
Ford, H ;
Lee, K .
DIGESTIVE DISEASES AND SCIENCES, 2001, 46 (05) :1127-1132