Amperometric glucose biosensor based on gold nanorods and chitosan comodified Au electrode

被引:0
作者
LI YingyingabWANG XiaoxiaaDONG XiangaHE Zhipengaand ZHANG Haiqianac a College of Material Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing China b Kunming Fire Service Training SchoolKunming China c Jiangsu Laboratory for Biomaterials and DevicesSoutheast UniversityNanjing China [210016 ,650208 ,210096 ]
机构
关键词
biochemistry; glucose biosensor; enzyme immobilization; gold nanorod;
D O I
暂无
中图分类号
TP212.3 [生物传感器、医学传感器];
学科分类号
080202 ;
摘要
Au nanorods were prepared by a seeding growth approach and used in fabricating the nanorod-enhancing glucose biosensor.The high affinity of chitosan for Au nanorods associated with its amino groups resulted in the formation of a layer of Au nanorods on the surface of Au electrode.It served as an intermediator to retain high efficient and stable immobilization of the enzyme.The performance of biosensors was investigated by cyclic voltammetry (CV),in the presence of artificial redox mediator,ferrocenecarboxaldehyde.The biosensor had a fast response to glucose,and the response time was less than 10 s.The results indicated that the gold nanorods could enhance the current response to glucose.The detection limits of glucose can reach 10 mM,and the Michaelis-Menten constant K mapp is 13.62 mM.
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页码:238 / 242
页数:5
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