银染PCR-SSCP检测大肠癌p53基因突变

被引：7
作者：
欧启水
王灏
沈仲毅
陆佩华
周光炎
机构：
[1] 上海第二医科大学!上海市免疫学研究所　
[2] 附属瑞金医院外科
来源：
中国实验诊断学 | 1997年 / 05期
关键词：
p53基因; 单链构象多态性; 基因突变; 大肠癌; 银染;
D O I：
暂无
中图分类号：
R735.3 [肠肿瘤];
学科分类号：
摘要：
采用银染ＰＣＲ－ＳＳＣＰ法检测肠癌细胞株及３０例新鲜肠癌Ｐ５３基因突变。结果发现ＬＯＶＯ细胞ｐ５３基因外显子５、６、７发生突变，ＬＳ１７４Ｔ细胞Ｐ５３基因的外显子５、８发生突变，３０例新鲜肿瘤标本ｐ５３基因外显子５、６、７、８的突变例数各为４、０、４、４例。大肠癌Ｐ５３基因突变热点为外显子５、７、８。
引用
收藏
页码：35 / 37
页数：3
相关论文
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