Studies on Electrochemical Behavior of Bleomycin and Its Interaction with DNA at a Co/GC Ion Implantation Modified Electrode

Electrochemical investigation of Bleomycin (BLM) and its interaction with DNA at a Co/GC ion implantation modified electrode was reported for the first time. The electrochemical behavior of BLM at Co/GC modified electrode has been studied by linear sweep and cyclic voltammetry, The reaction of BLM with DNA formed an electrochemically nonactive complex, which resulted in a decrease in the BLM equilibrium concentration and its reduction current. The decrease in peak current was proportional to DNA concentration and could be used to determination DNA concentration. The equilibrium constant of DNA-BLM complex β was determined to be 5.16×10 16. The experiments of AES and XPS showed that Co was surely implanted into the surface of GCE(glassy carbon electrode) and the depth distribution of Co was in good agreement with Gaussian normal distribution; the implanted Co at GCE improved the electrocatalytic activity.