BACKGROUND:Alterations in DNA methylation occur during the pathogenesis of human tumors.In this study, we investigated the influence of DNA methyltransferase 3b(DNMT3b)on fragile histidine trial(FHIT)expression and on DNA methylation of the FHIT promoter region in the hepatoma cell line SMMC-7721. METHODS:DNMT3b siRNA was used to down-regulate DNMT3b expression.DNMT3b and FHIT proteins were determined by Western blotting.Methylation-specific PCR was used to analyze the methylation status of the FHIT gene. RESULTS:After DNMT3b siRNA transfection,the expression of DNMT3b was inhibited in SMMC-7721 cells,and the expression of FHIT was significantly higher than that in the control group.There was no significant difference in methylation status between the DNMT3b siRNA transfected cells and control cells. CONCLUSION:DNMT3b may play an important role in regulation of FHIT expression in hepatoma SMMC-7721 cells,but not through methylation of the FHIT promoter.
机构:
Southeast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Minist Educ, Key Lab Dev Genes & Human Dis, Nanjing, Jiangsu, Peoples R ChinaSoutheast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Lao, Yingbin
Wu, Huazhang
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Southeast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Minist Educ, Key Lab Dev Genes & Human Dis, Nanjing, Jiangsu, Peoples R ChinaSoutheast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Wu, Huazhang
Zhao, Chengchegn
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Southeast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Minist Educ, Key Lab Dev Genes & Human Dis, Nanjing, Jiangsu, Peoples R ChinaSoutheast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Zhao, Chengchegn
Wu, Qunying
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Guilin Med Univ, Inst Biotechnol, Guilin 541004, Guangxi, Peoples R ChinaSoutheast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Wu, Qunying
Qiao, Fengchang
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Southeast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Minist Educ, Key Lab Dev Genes & Human Dis, Nanjing, Jiangsu, Peoples R ChinaSoutheast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Qiao, Fengchang
Fan, Hong
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Southeast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
Minist Educ, Key Lab Dev Genes & Human Dis, Nanjing, Jiangsu, Peoples R ChinaSoutheast Univ, Sch Med, Dept Med Genet & Dev Biol, 87 Dingjiaqiao, Nanjing 210009, Jiangsu, Peoples R China
机构:
Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R ChinaNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
Pan, C.
Jia, W.
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Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R ChinaNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
Jia, W.
Wu, X.
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Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R ChinaNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
Wu, X.
Zhao, H.
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Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R ChinaNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
Zhao, H.
Liu, S.
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Hanzhou Vocat & Teaching Coll, Dept Chem & Engn, Hangzhou 310018, Zhejiang, Peoples R ChinaNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
Liu, S.
Lei, C.
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Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R ChinaNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
Lei, C.
Lan, X.
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Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R ChinaNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
Lan, X.
Chen, H.
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Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R ChinaNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
Chen, H.
JOURNAL OF ANIMAL AND PLANT SCIENCES,
2013,
23
(02):
: 380
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387