Influence of DNA methyltransferase 3b on FHIT expression and DNA methylation of the FHIT promoter region in hepatoma SMMC-7721 cells

被引:0
|
作者
Jia-Xiang Wang
机构
基金
中国国家自然科学基金;
关键词
liver neoplasms; DNA methyltransferase 3b; fragile histidine trial; methylation; gene expression;
D O I
暂无
中图分类号
R735.7 [肝肿瘤];
学科分类号
100214 ;
摘要
BACKGROUND:Alterations in DNA methylation occur during the pathogenesis of human tumors.In this study, we investigated the influence of DNA methyltransferase 3b(DNMT3b)on fragile histidine trial(FHIT)expression and on DNA methylation of the FHIT promoter region in the hepatoma cell line SMMC-7721. METHODS:DNMT3b siRNA was used to down-regulate DNMT3b expression.DNMT3b and FHIT proteins were determined by Western blotting.Methylation-specific PCR was used to analyze the methylation status of the FHIT gene. RESULTS:After DNMT3b siRNA transfection,the expression of DNMT3b was inhibited in SMMC-7721 cells,and the expression of FHIT was significantly higher than that in the control group.There was no significant difference in methylation status between the DNMT3b siRNA transfected cells and control cells. CONCLUSION:DNMT3b may play an important role in regulation of FHIT expression in hepatoma SMMC-7721 cells,but not through methylation of the FHIT promoter.
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页码:273 / 277
页数:5
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