An Enzyme Electrode Based on the Covalently Binding of Horseradish Peroxidase to a Self-assembling Monolayer Modified Gold Electrode

A new procedure for developing high performance enzyme electrode is described. Theprocedure is based on the covalently binding of horseradish Peroxidase to a self assemblingmonolayer on a gold electrode. An enhanced catalytic current is observed with addihon of hydro-gen peroxide into phosphate buffer solution (PBS) in the presence of mediators. The electrodeexhibits high sensihvity and a wide linear range to hydrogen peroxide.