The YAC Contig Construction for Human X Chromosome Xp21. 3—11. 3

被引：0

作者：

缪为民 ^{[1
]}

机构：

[1] Institute of Genetics Fudan University Shanghai 200433 P. R. China

来源：

HighTechnologyLetters | 1997年 / 02期

基金：

中国国家自然科学基金;

关键词：

Human genome; X chromosome; YAC contig; STS map;

D O I：

暂无

中图分类号：

R596 [遗传性疾病];

学科分类号：

1002 ; 100201 ;

摘要：

The YAC contig construction has been done for the Human X chromosome short armXp21.3—p11.3, a region which contains several genetic disease gene loci and is of highlybiomedical importance. Using known probes(OTC, DXS166, DMDcDNA) and STS markersof this region, YAC screenings are performed by both YAC colony in situ hybridization andPCR methods. Totally 55 YACs are obtained from the YAC libraries of CEPH, ICRF andthe Institute. The size determination, the analysis of 26 pairs of microsatelite STS, the single copy probe hybridization and the Alu-PCR fingerprinting are performed for these YACs.The mapping of these YACs is performed, and finally, 6 YAC contigs in Xp21.3—11.3 are obtained, covering about 15 Mb. This work will greatly facilitate the positional cloning of disease genes or the genome sequencing in this important region.

引用

页码：94 / 99

页数：6

共 12 条

[1] Willard H F. Report of the fifth international workshop on human X chromosome mapping . 1994
[2] Foote S et al. Science . 1992
[3] Albertertsen H M et al. Proceedings of the National Academy of Sciences of the United States of America . 1990
[4] Collins F,Galas D. Science . 1993
[5] Baldini A et al. Genomics . 1992
[6] Chai J H,Gu Y H. The Acta of Genetics . 1993
[7] Miao W M,Chai J H. High Tech. letters . 1993
[8] Olson M V et al. Science . 1989
[9] Nagaraja R et al. Nucleic Acids Research . 1989
[10] Wei Y et al. The Acta of Biophysics and Biochemistry . 1995

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