DNA vaccine pCD-Sj32 and its efficacy of protective immunity against infection of Schistosoma japonicum

被引：0

作者：

石佑恩

谢来平

李传明

韩家俊

机构：

来源：

中华医学杂志(英文版) | 1999年 / 08期

关键词：

Schistosoma japonicum · DNA vaccine · protective immunity;

D O I：

暂无

中图分类号：

R392.1 [免疫生物学];

学科分类号：

100102 ;

摘要：

Objective To study protective immunity afforded by murine immunization with DNA vaccine of Schistosoma japonicum (S. japonicum) as measured by reduction in worm burden and host antibody, cytokines.Methods DNA vaccine pCD Sj32 was constructed. identificated and expressed. pCD Sj32 could induce substantial protective immunity against infection of S. japonicum in BALB/c mice. The best efficacy can be produced with one injection of 100?μg DNA into the quadriceps muscle, combined with challenge for 8 weeks after immunization. T lymphocyte subsets of CD 8 +, IL 2. TNF and IFN γ of experimental animal could play important roles in regulating immune functions of schistosomiasis. Results High titre of specific antibody IgG could be induced by vaccinated with pCD Sj32, and antibody can mediate macrophage to produce ADCC effects in vitro. Conclusion pCD Sj32 may represent a new approach to developing subunit vaccine.

引用

页码：5 / 8

页数：4

共 4 条

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