Measurement of interaction force between RGD-peptide and Hela cell surface by optical tweezers

被引：0

作者：

钟敏成 ^{[1
,2
]}

薛国胜 ^{[1
]}

周金华 ^{[1
]}

王自强 ^{[1
]}

李银妹 ^{[1
,2
,3
]}

机构：

[1] Department of Optics and Optical Engineering, University of Science and Technology of China

[2] Anhui Key Laboratory for Optoelectronic Science and Technology

[3] Hefei National Laboratory for Physical Sciences at the Microscale

来源：

ChineseOpticsLetters | 2012年 / 10卷 / 10期

关键词：

Hela; RGD; Measurement of interaction force between RGD-peptide and Hela cell surface by optical tweezers; cell;

D O I：

暂无

中图分类号：

R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Since RGD peptides (R: arginine; G: glycine; D: aspartic acid) are found to promote cell adhesion, they are modified at numerous materials surface for medical applications such as drug delivery and regenerative medicine. Peptide-cell surface interactions play a key role in the above applications. In this letter, we study the adhesion force between the RGD-coated bead and Hela cell surface by optical tweezes. The adhesion is dominated by the binding of α5β1 and RGD-peptide with higher adhesion probability and stronger adhesion strength compared with the adhesion of bare bead and cell surface. The binding force for a single α5β1 -GRGDSP pair is determined to be 16.8 pN at a loading rate of 1.5 nN/s. The unstressed off-rate is 1.65 × 10-2s-1 and the distance of transition state for the rigid binding model is 3.0 nm.

引用

页码：57 / 59

页数：3