Identification of the HAK gene family reveals their critical response to potassium regulation during adventitious root formation in apple rootstock

被引：4

作者：

Muhammad Mobeen Tahir ^{[1
]}

Lu Tong ^{[1
]}

Lingling Xie ^{[1
]}

Tong Wu ^{[1
]}

Muhammad Imran Ghani ^{[1
]}

Xiaoyun Zhang ^{[2
]}

Shaohuan Li ^{[1
]}

Xiuhua Gao ^{[1
]}

Leeza Tariq ^{[3
]}

Dong Zhang ^{[1
]}

Yun Shao ^{[1
]}

机构：

[1] Yangling Sub-Center of National Center for Apple Improvement,College of Horticulture,Northwest Agriculture & Forestry University

[2] The Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group,College of Agriculture,Shihezi University

[3] Centre of Excellence in Molecular Biology,University of the Punjab

来源：

HorticulturalPlantJournal | 2023年 / 9卷 / 01期

关键词：

D O I：

暂无

中图分类号：

S661.1 [苹果];

学科分类号：

090201 ;

摘要：

Adventitious root formation is a bottleneck during vegetative proliferation.Potassium （K+） is an essential macronutrient for plants.K+accumulation from the soil and its distribution to the different plant organs is mediated by K+transporters named K+transporter （KT）,K+uptake （KUP）,or high-affinity K+（HAK）.This study aimed to identify members of the HAK gene family in apples and to characterize the effects of K+supply on adventitious root formation and on the expression of HAK genes and the genes that putatively control auxin transport,signaling,and cell fate during adventitious root formation.In this study,34 HAK genes （MdHAKs） were identified in the apple （Malus×domestica‘Golden Delicious’） genome.A phylogenetic analysis divided MdHAKs into four clusters （Ⅰ,Ⅱ,Ⅲ,and Ⅳ）,comprising 16,1,4,and 13 genes,respectively.The syntenic relationships revealed that 62.5%of the total MdHAK genes arise from genomic duplication events.Chromosome location,domain structure,motif analysis,and physico-chemical characteristics were subsequently investigated.Furthermore,the application of K+indicated the emergence of adventitious roots at 8 d and produced more adventitious roots at 16 d than the K+-free control （CK） treatment.In addition,various MdHAKs showed root-specific expression in B9 apple rootstock stem cuttings and enhanced expression during the initiation and emergence stages of adventitious root formation in response to K+treatment.Additionally,K+treatment enhanced the expression levels of MdPIN1,MdPIN2,and MdAUX1.Further data indicated that a higher expression of MdWOX11,MdLBD16,and MdLBD29 and of cell cycle-related genes contributed to the auxin-stimulated adventitious root formation in response to K+.

引用

页码：45 / 59

页数：15

共 70 条

[1]

The Growth-Promoting Mechanism of Brevibacillus laterosporus AMCC100017 on Apple Rootstock Malus robusta[J]. Xiaona Wang,Jiucheng Zhang,Xiaofei Wang,Jianping An,Chunxiang You,Bo Zhou,Yujin Hao.Horticultural Plant Journal. 2022(01)

[2] 矮化中间砧苹果施氮位置对细根分布、氮素吸收和产量品质的影响 [J].

刘照霞 ;

邢玥 ;

吴晓娴 ;

田歌 ;

朱占玲 ;

葛顺峰 ;

姜远茂 .

园艺学报, 2021, 48 (02) :219-232

[3] 双主干并棒树形对矮化自根砧苹果幼树生长和结果的影响 [J].

马小龙 ;

马豆豆 ;

史继东 ;

韩明玉 ;

杨伟伟 ;

张东 .

园艺学报, 2020, 47 (03) :541-550

[4] 矮化中间砧‘宫藤富士’苹果栽植密度对树体生长、冠层光照和果实产量的影响 [J].

李民吉 ;

张强 ;

李兴亮 ;

周贝贝 ;

高木旺 ;

杨雨璋 ;

周佳 ;

张军科 ;

魏钦平 .

园艺学报, 2020, 47 (03) :421-431

[5] 低钾胁迫对苹果砧木M9T337幼苗光合荧光特性及13C吸收分配的影响 [J].

徐新翔 ;

侯昕 ;

王芬 ;

贾志航 ;

葛顺峰 ;

姜远茂 .

园艺学报, 2020, 47 (03) :529-540

[6]

Progress of Apple Rootstock Breeding and Its Use[J]. Yi Wang,Wei Li,Xuefeng Xu,Changpeng Qiu,Ting Wu,Qinping Wei,Fengwang Ma,Zhenhai Han.Horticultural Plant Journal. 2019(05)

[7]

Genome-wide Identification and Expression of ARF Gene Family during Adventitious Root Development in Hot Pepper（Capsicum annuum）[J]. ZHANG Huanxin,CAO Ning,DONG Chunjuan,SHANG Qingmao.Horticultural Plant Journal. 2017(04)

[8] Insights into Factors Controlling Adventitious Root Formation in Apples [J].