Initial function analysis of a novel erythroid differentiation related gene EDRF1

被引:0
作者
王敦成
黎燕
沈倍奋
机构
关键词
HS2 binding proteins; hemopoiesis; cell clongenicity; expression pattern;
D O I
暂无
中图分类号
Q343.1 [基因理论];
学科分类号
071007 ; 090102 ;
摘要
Erythroid differentiation depends on the establishment of specific patterns of gene expression. Hypersensitive site 2 (HS2, serving as a major enhancer of globin genes)-binding proteins may be involved in its natural open chromosomal environment formation. Previously we prepared monoclonal antibodies against HS2-binding nuclear proteins of terminal differentiated erythroid cells. By utilizing the monoclonal antibodies, we screened λ-gt11 human fetal liver cDNA expression library and obtained one cDNA clone, which was named erythroid differentiation related gene (EDRF1, Genbank accession number AF040247) , encompassing an entire open reading frame. We investigated the expression pattern of EDRF1 by RT-PCR technique. And a clue to the function of EDRF1 has been found from confirmation of high levels of EDRF1 mRNA in differentiated K562 and human fetal liver tissue. To illuminate the function of EDRF1 in K562 cells, sense and antisense EDRF1 constructs were prepared and transfected into K562 cells, α-glob
引用
收藏
页码:489 / 496
页数:8
相关论文
共 4 条
[1]  
cDNA cloning and function analysis of two novel erythroid differentiation related genes[J]. 王鑫 ,王敦成 ,陈兴 ,胡美茹 ,王建安 ,黎燕 ,郭宁 ,沈倍奋.Science in China(Series C:Life Sciences). 2001(01)
[2]   红细胞终末分化期出现与珠蛋白基因表达增强子HS2序列特异结合的蛋白质因子 [J].
王鑫,刘丕旭,章静波,薛社普 .
解剖学报, 1994, (04) :379-384
[3]  
The making of an erythroid cell[J] . Anna Rita Migliaccio,Giovanni Migliaccio.Biotherapy . 1998 (4)
[4]   GENERATION OF COMMITTED ERYTHROID BFU-E AND CFU-E PROGENITORS DOES NOT REQUIRE ERYTHROPOIETIN OR THE ERYTHROPOIETIN RECEPTOR [J].
WU, H ;
LIU, X ;
JAENISCH, R ;
LODISH, HF .
CELL, 1995, 83 (01) :59-67