Baculovirus ETL promoter acts as a shuttle promoter between insect cells and mammalian cells

<正> Aim:To identify a shuttle promoter that can mediate gene expression in bothinsect cells and mammalian cells to facilitate the development of a baculovirusvector-based mammalian cell gene delivery vehicle.Methods:Recombinantbaculoviruses carrying the β-galactosidase reporter gene under the control of anearly to late(ETL)promoter of the Autographa californica multiple nuclear poly-hedrosis virus(AcMNPV)or a cytomegalovirus immediate early promoter(CMVpromoter)were constructed.COS1,HeLa,CHO-K1,hFobl.19,and MCF-7 mam-malian cells were tested for the expression of β-galactosidase.Results:ETLpromoter activity was higher in bone-derived hFob 1.19 than in COS1,HeLa,CHO-K1,or MCF-7 mammalian cells.The transient plasmid transfection assay indi-cated that ETL promoter activity in mammalian cells was dependent on baculovirusgene expression.Conclusion:ETL promoter activity in mammalian cells isbaculovirus gene expression-dependent,and the shuttle promoter will facilitatethe application of baculovirus expression vectors in mammalian cell expressionsystems and for gene therapy.