Interaction of Chelerythrine Choloride in Acid Buffer with Calf-thymus DNA

被引：0
|
作者：
Yan Jing SU
机构：
来源：
ChineseChemicalLetters | 2006年 / 05期
关键词：
Chelerythrine; CT-DNA; intercalation; thermodynamic;
D O I：
暂无
中图分类号：
Q523 [脱氧核糖核酸（DNA）];
学科分类号：
071010 ; 081704 ;
摘要：
The binding of chelerythrine (CHE) iminium to calf-thymus (CT) DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polarization, and the perturbation of the CD spectra of DNA. These changes are characteristic of intercalation. Scatchard plots constructed from fluorescence titration data gave a binding constant of 5.77×105 and a binding site size of 3,4 base pairs at 25℃. The intercalative interaction is exothermic with a Van’t Hoff enthalpy of -33.7 kJ/mol. In addition, *G =-32.9 kJ/mol, and *S =-2.9 J/mol/K. The intercalation of CHE into the DNA helix is enthalpy-driven.
引用
收藏
页码：691 / 694
页数：4
相关论文
共 50 条
  • [21] Fluorescence Interaction and Determination of Calf Thymus DNA with Two Ethidium Derivatives
    Nuriye Akbay
    Zeynel Seferoğlu
    Elmas Gök
    Journal of Fluorescence, 2009, 19 : 1045 - 1051
  • [22] Multi-spectroscopic and molecular simulation methods of analysis to explore the mode of binding of Mebendazole drug with calf-thymus DNA
    Pavithra, K.
    Priyadharshini, R. Durga
    Vennila, K. N.
    Elango, Kuppanagounder P.
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2023, 300
  • [23] Bioelectrochemical sensing of promethazine with bamboo-type multiwalled carbon nanotubes dispersed in calf-thymus double stranded DNA
    Primo, Emiliano N.
    Belen Oviedo, M.
    Sanchez, Cristian G.
    Rubianes, Maria D.
    Rivas, Gustavo A.
    BIOELECTROCHEMISTRY, 2014, 99 : 8 - 16
  • [24] Determination of the DNA binding properties of a novel PARP inhibitor MK-4827 with calf-thymus DNA by molecular simulations and detailed spectroscopic investigations
    Yang, Hongqin
    Zeng, Qingle
    He, Ze
    Wu, Di
    Li, Hui
    NEW JOURNAL OF CHEMISTRY, 2019, 43 (17) : 6702 - 6711
  • [25] Interaction of palladium(II) coordination compounds with calf thymus DNA and their antibacterial activity
    Patel, Mohan N.
    Dosi, Promise A.
    Bhatt, Bhupesh S.
    INORGANIC CHEMISTRY COMMUNICATIONS, 2012, 21 : 61 - 64
  • [26] Solvent Effects on the Kinetics of the Interaction of 1-Pyrenecarboxaldehyde with Calf Thymus DNA
    Secco, Fernando
    Venturini, Marcella
    Biver, Tarita
    Sanchez, Francisco
    Prado-Gotor, Rafael
    Grueso, Elia
    JOURNAL OF PHYSICAL CHEMISTRY B, 2010, 114 (13) : 4686 - 4691
  • [27] Study on the interaction between methylene violet and calf thymus DNA by molecular spectroscopy
    Dong, C
    Wei, YX
    Wei, YL
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2005, 174 (01) : 15 - 22
  • [28] Spectroscopic studies on the interaction of sodium benzoate, a food preservative, with calf thymus DNA
    Zhang, Guowen
    Ma, Yadi
    FOOD CHEMISTRY, 2013, 141 (01) : 41 - 47
  • [29] Interaction of antioxidant flavonoids with calf thymus DNA analyzed by spectroscopic and electrochemical methods
    Hegde, Ashwini H.
    Prashanth, S. N.
    Seetharamappa, J.
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2012, 63 : 40 - 46
  • [30] Study on the interaction of triadimenol with calf thymus DNA by multispectroscopic methods and molecular modeling
    Zhang, Yepeng
    Zhang, Guowen
    Fu, Peng
    Ma, Yadi
    Zhou, Jia
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2012, 96 : 1012 - 1019
12345