GenerationofnewglutinousricebyCRISPR/Cas9-targetedmutagenesisoftheWaxygeneinelitericevarieties

被引：1

作者：

Jinshan Zhang ^{[1
,2
]}

Hui Zhang ^{[1
,3
]}

Jos Ramn Botella ^{[4
]}

JianKang Zhu ^{[1
,3
]}

机构：

[1] Shanghai Center for Plant Stress Biology, and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences

[2] University of Chinese Academy of Sciences

[3] Department of Horticulture and Landscape Architecture, Purdue University

[4] Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland

来源：

Journal of Integrative Plant Biology | 2018年 / 60卷 / 05期

关键词：

Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties; Figure; AC;

D O I：

暂无

中图分类号：

S511 [稻];

学科分类号：

0901 ;

摘要：

In rice, amylose content（AC） is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats（CRISPR）/CRISPR-associated 9（Cas9） to introduce a loss-of-function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.

引用

页码：369 / 375

页数：7

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