P53 Regulation of Leukemia Cells with the Blockage of MDM2 by Antisense Oligonucleotides

被引：0

作者：

房明浩 ^{[1
]}

纪学梅 ^{[2
]}

汤屹 ^{[3
]}

刘文励 ^{[3
]}

机构：

[1] Department of Internal Emergency,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan ,China

[2] Department of Chemotherapy and Radiation,Zhongnan Hospital,Wuhan University,Wuhan ,China

[3] Department of Hematology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan ,China

来源：

华中科技大学学报(医学英德文版) | 2006年 / 04期

关键词：

leukemia; oncogene; antisense; oligonucleotides; gene therapy;

D O I：

暂无

中图分类号：

R733.7 [白血病];

学科分类号：

100214 ;

摘要：

The changes of expression and function of MDM2 and P53 by MDM2 specific antisense oligonucleotides were investigated in HL60 cells. Cells were divided into control group, AS group (MDM2 specific antisense oligonucleotides group), cisplatin group, and combined treatment group. FCM analysis and Western blot and RT-PCR were used to estimate apoptosis and the expression of MDM2 and P53. Our results showed that the transfection of MDM2 specific antisense oligonucleo- tides obviously inhibited MDM2 expression (P<0.01) and increased the expression of P53 (P<0.05). Apoptosis rate were reduced by MDM2 specific antisense oligonucletides and cisplatin (P<0.01). It is concluded that MDM2 specific antisense oligonucletides can inhibit the expression of MDM2, induce the expression of P53 and increase the apoptosis of leukemia cells after chemotherapy.

引用

页码：414 / 416

页数：3