Cloning of the Aspergillus awamori glucoamylase gene and expression in Saccharomyces cerevisiae

被引：0
|
作者：
WANG Yongji
机构：
来源：
ChineseScienceBulletin | 1998年 / 10期
关键词：
Aspergillus awamori; glucoamylase; gene cloning and expression; Saccharomyces cerevisiae;
D O I：
暂无
中图分类号：
Q785 [转化及克隆];
学科分类号：
071007 ; 0836 ; 090102 ;
摘要：
The A. awamori glucoamylase I gene was obtained by RT_PCR and inserted into the plasmid pAC1. We constructed an expression vector pAC1_GA, which was used to transform the yeast S. cerevisiae AS 2 1364 without auxotrophic marker. The transformant secreted glucoamylase into the medium efficiently and degraded starch. The glucoamylase activity of the culture filtrate is up to 8 4 U/mL.
引用
收藏
页码：855 / 859
页数：5
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