We investigated the eff ects of temperature(14,20,and 22°C),salinity(5,10,15,20,and 25),and body length(0.83,1.17,1.49,and 2.33 mm)on the energy budget of Daphniopsis tibetana Sars in the laboratory.The results demonstrated no diff erence in D.tibetana assimilation efficiency(AE)with values of 51.59%–56.22% at the trial temperatures.Gross growth efficiency(K)and net growth efficiency(K)were 28.78% and 55.71%,respectively,at 14°C.Daphniopsis tibetana energy conversion efficiency(AE,K,and K)was higher at salinities of 10–20,with values of 72.80%–77.31%,42.50%–50.42%,and 58.43%–65.02%,respectively.Daphniopsis tibetana energy conversion efficiency was highest when body length was 1.17–1.19 mm,with values of 83.70%–84.73%,50.11%–50.81%,and 59.13%–60.71%,respectively.The results of this study can provide a reference for large-scale cultivation use of D.tibetana in the further.