Cloning,Sequencing and Characterization of 3-Hydroxybutyrate Dehydrogenase Encoding Gene(bdh A)in Bradyrhizobium japonicum USDA110 Strain

被引：0

作者：

Charles Trevor C

机构：

[1] Canada

[2] Waterloo N2L 3G1

[3] Biology Department

[4] University of Waterloo

来源：

AgriculturalSciencesinChina | 2002年 / 09期

关键词：

Bradyrhizobium japonicum; bdhA gene; Bdh; PHB;

D O I：

暂无

中图分类号：

Q785 [转化及克隆];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

The current study describes the molecular characterization of a clone which can restore the ability of bdhA mutant strains NGRPA2 and Rm11107 to utilize 3-hydroxybutyrate as a sole carbon source (Hbu+ ). This clone was screened out by complementation experiment from Bradyrhizobium japonicum US-DAI 10 genomic library, and the presence of bdhA gene in the clone was verified by Bdh assay and Southern blot analysis. Furthermore, the entire sequence of bdhA gene was sequenced and the sequence was deposited in

引用

页码：89 / 97

页数：9

共 21 条

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