Resolution enhancement in hydrophobic interaction chromatography via electrostatic interactions

被引：1
|
作者：
Dong Gao [1 ]
Fu-Chun Tan [1 ]
Wen-Peng Wang [1 ]
Li-Li Wang [1 ]
机构：
[1] Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education,Shaanxi Key Laboratory of Modern Separation Science,Institute of Modern Separation Science,Northwest University
来源：
ChineseChemicalLetters | 2013年 / 24卷 / 05期
关键词：
Hydrophobic interaction chromatography; Electrostatic interaction; Resolution; Hen egg white;
D O I：
暂无
中图分类号：
TQ322 [合成树脂及塑料];
学科分类号：
摘要：
In this work,a new type of hydrophobic stationary phase that provide electrostatic interactions with analytes was developed by bondingβ-phenylethylamine as a functional ligand to silica.This stationary phase can separate proteins with similar hydrophobicity that traditional hydrophobic resins cannot.Hen egg white was separated to examine the selectivity.The results show that the introduced electrostatic interactions are an important factor for the resolution enhancement and the new resin could have important applications in separation and purification of biological macromolecules.
引用
收藏
页码：419 / 421
页数：3
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