Effect of Y-27632 on the cultured retinal neurocytes of rats

被引：0

作者：

Pei-Li Feng ^{[1
]}

Jing Wang ^{[1
]}

Zi-Jian Yang ^{[1
]}

Xiao-Hong Liu ^{[1
]}

Yi-Sheng Zhong ^{[1
]}

机构：

[1] Department of Ophthalmology,Ruijin Hospital Affiliated Medical School,Shanghai Jiaotong University

来源：

International Journal of Ophthalmology | 2013年 / 01期

基金：

中国国家自然科学基金;

关键词：

Y-27632; retinal neurocytes; cell culture; neurites;

D O I：

暂无

中图分类号：

R774.1 [视网膜疾病];

学科分类号：

100212 ;

摘要：

AIM:To investigate the effect of Y-27632 on the survival and neurite outgrowth of the cultured retinal neurocytes. METHODS:After the postnatal day 2-3, Sprague-Dawley retinal neurocytes were cultured for 48 hours, the culture media was replaced with serum-free media (control group) and serum-free media contained 30μmol/L Y-27632 (Y-27632 group), and the cells were continually cultured another 48 hours. The cultured retinal neurocytes were identified with anti-neuron specific enolase (NSE) immunocytochemistry. The survival state of those cells was estimated by MTT assay, and the neurite outgrowth of those cells was evaluated by the computerized image-analysis system. RESULTS:Compared with the control group, the absorbance values of cells survival in Y -27632 group increased 12.90% and 33.33% respectively after 72 and 96 hours culture. Y-27632 had no significant effect on the diameter of cultured retinal neurocytes. Compared with the control group, Y-27632 induced a stable improvement of neurite outgrowth of retinal neurocytes after 72 and 96 hours culture (P =0.001). CONCLUSION:Y-27632 could promote the survival and neurite outgrowth of the early postnatal cultured retinal neurocytes.

引用

页码：15 / 18

页数：4

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