长江流域禾谷镰孢菌群部分菌株系统发育学、产毒素化学型及致病力研究

被引:19
作者
李伟 [1 ]
胡迎春 [1 ,2 ]
陈莹 [1 ,3 ]
张爱香 [1 ]
陈怀谷 [1 ]
机构
[1] 江苏省农业科学院植物保护研究所
[2] 南京农业大学植物保护学院
[3] 南京农业大学生命科学学院
关键词
Fusariumasi aticum; 3-乙酰脱氧雪腐镰孢菌烯醇; 强致病力菌株;
D O I
10.13346/j.mycosystema.2010.01.012
中图分类号
S435.12 [麦类病虫害];
学科分类号
090401 ; 090402 ;
摘要
从采集自长江流域引起小麦赤霉病的禾谷镰孢菌群(Fusarium graminearum clade)菌株中选取了31株,扩增并测定了这些菌株的EF-1α(translation elongation factor)、PHO(phosphate permease)基因序列,利用相关软件进行了系统发育分析。对这些菌株的产毒素化学型进行了分子检测。同时,用两个小麦品种(扬麦158和安农8455)测定了菌株的致病力。系统发育分析表明绝大多数菌株与F.asiaticum聚为一枝,只有一个菌株11027与F.graminearum聚类。30株F.asiaticum中有24株产脱氧雪腐镰孢菌烯醇(deoxynivalenol,DON)和3-乙酰脱氧雪腐镰孢菌烯醇(3-acetyldeoxynivalenol,3-AcDON),另外6株产雪腐镰孢菌烯醇(Nivalenol,NIV)。一株F.graminearum菌株11027产脱氧雪腐镰孢菌烯醇(DON)和15-乙酰脱氧雪腐镰孢菌烯醇(15-acetyldeoxynivalenol,15-AcDON)。在扬麦158上,菌株间的致病力分化较为明显,产NIV毒素的菌株致病力普遍较弱,强致病力的菌株都产3-AcDON毒素。结果表明在我国长江流域,产3-AcDON毒素的F.asiaticum是引起小麦赤霉病的优势种群,中抗赤霉病的小麦品种扬麦158可以有效评价菌株的致病力强弱。
引用
收藏
页码:51 / 58
页数:8
相关论文
共 8 条
[1]   基于Windows的核酸序列分析软件的开发 [J].
黄骥 ;
张红生 .
生物信息学, 2004, (01) :13-17
[2]   Global molecular surveillance reveals novel Fusarium head blight species and trichothecene toxin diversity [J].
Starkey, David E. ;
Ward, Todd J. ;
Aoki, Takayuki ;
Gale, Liane R. ;
Kistler, H. Corby ;
Geiser, David M. ;
Suga, Haruhisa ;
Toth, Beata ;
Varga, Janos ;
O'Donnell, Kerry .
FUNGAL GENETICS AND BIOLOGY, 2007, 44 (11) :1191-1204
[3]  
Determination of the trichothecene mycotoxin chemotypes and associated geographical distribution and phylogenetic species of the Fusarium graminearum clade from China[J] . Jing-Bo Zhang,He-Ping Li,Fu-Jun Dang,Bo Qu,Yu-Bin Xu,Chun-Sen Zhao,Yu-Cai Liao.Mycological Research . 2007 (8)
[4]   A new species of Epichloe symbiotic with Chinese grasses [J].
Li, Wei ;
Ji, Yan-Ling ;
Yu, Han-Shou ;
Wang, Zhi-Wei .
MYCOLOGIA, 2006, 98 (04) :560-570
[5]   Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum [J].
Li, HP ;
Wu, AB ;
Zhao, CS ;
Scholten, O ;
Löffler, H ;
Liao, YC .
FEMS MICROBIOLOGY LETTERS, 2005, 243 (02) :505-511
[6]  
Heading for disaster: Fusarium graminearum on cereal crops[J] . RUBELLA S.GOSWAMI,H. CORBYKISTLER.Molecular Plant Pathology . 2004 (6)
[7]   Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade [J].
O'Donnell, K ;
Ward, TJ ;
Geiser, DM ;
Kistler, HC ;
Aoki, T .
FUNGAL GENETICS AND BIOLOGY, 2004, 41 (06) :600-623
[8]   Determination of deoxynivalenol and nivalenol chemotypes of Fusarium culmorum isolates from England and Wales by PCR assay [J].
Jennings, P ;
Coates, ME ;
Turner, JA ;
Chandler, EA ;
Nicholson, P .
PLANT PATHOLOGY, 2004, 53 (02) :182-190