Signal transduction pathway of nitric oxide inducing PC12 cell death

被引:0
作者
罗成义
徐如祥
杨志林
机构
关键词
Transduction; signal; PC12; cells; Cell death; Nitric oxide;
D O I
暂无
中图分类号
R641.02 [];
学科分类号
1002 ; 100210 ;
摘要
Objective:: To study signal transduction pathway of nitric oxide inducing death of PC12 cells. Methods: Cell survival rate was measured with MTT assay, and caspase-3 activity with caspase-3 assay kits after PC12 cells were incubated with sodium nitroprusside (SNP), caspase-3 inhibitor Ⅱ plus SNP or p38 inhibitor-SB203580 plus SNP. Results: SNP induced death of PC12 cells in dose- and time-dependent manner and enhanced caspase-3 activity gradually. Both caspase-3 inhibitor Ⅱ and SB203580 reduced cell death, but SB203580 reduced caspase-3 activity significantly. Conclusions: NO may induce death of PC12 cells through activation of p38 and caspase-3.
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页码:22 / 24
页数:3
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Activation and cleavage ofcaspase-3in apopto-sis induced by experim entalcerebralischem ia. N am ura S,Zhu J,Fink K,etal. Journal of the Neurological Sciences . 1998