Characterization and mapping of a lesion mimic mutant in rice (Oryza sativa L.)

被引:1
作者
LIU Daofeng
Hebei Agriculture University
机构
关键词
Oryza sativa L; lesion mimic mutant; molecular marker; genetic mapping;
D O I
暂无
中图分类号
Q943 [植物细胞遗传学];
学科分类号
071007 ; 090102 ;
摘要
A rice initiation-type lesion mimic mutant (lmi) was identified, which was isolated from an indica rice Zhongxian 3037 through y radiation mutagenesis. Trypan blue staining and sterile culture revealed that the mutant spontaneously developed lesions on the leaves in a develop-mentally regulated and light-dependent manner. Genetic analysis indicated that the lesion mimic trait was controlled by a single resessive locus. Using public molecular markers and an F2 population derived from lmi and 93-11, we mapped the lmi locus to the short arm of chromosome 8, nearby the centromere, between two SSR markers RM547 and RM331. The genetic distance was 1.2 and 3.2 cM, respectively. Then according to the public rice genomic sequence between the two SSR markers, lmi was further finely tagged by three CAPS markers: C4135-8, C4135-9 and C4135-10. And lmi locus was a co-segregated with marker C4135-10, providing a starting point for lmi gene cloning.
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页码:892 / 896
页数:5
相关论文
共 7 条
  • [1] Morphology and inheritance of a new rice leaf death mutant
    CHENG Zhukuan and GU Mingfiong
    [J]. Chinese Rice Research Newsletter, 1998, (03) : 2 - 2
  • [2] Fine genetic mapping and physical delimitation of the lesion mimic gene Spl11 to a 160-kb DNA segment of the rice genome
    Zeng, L
    Yin, Z
    Chen, J
    Leung, H
    Wang, GL
    [J]. MOLECULAR GENETICS AND GENOMICS, 2002, 268 (02) : 253 - 261
  • [3] A novel suppressor of cell death in plants encoded by the Lls1 gene of maize
    Gray, J
    Close, PS
    Briggs, SP
    Johal, GS
    [J]. CELL, 1997, 89 (01) : 25 - 31
  • [4] A novel zinc finger protein is encoded by the arabidopsis LSD1 gene and functions as a negative regulator of plant cell death
    Dietrich, RA
    Richberg, MH
    Schmidt, R
    Dean, C
    Dangl, JL
    [J]. CELL, 1997, 88 (05) : 685 - 694
  • [5] The Barley Mlo Gene: A Novel Control Element of Plant Pathogen Resistance[J] . Cell . 1997 (5)
  • [6] Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice ( Oryza sativa L.)[J] . O. Panaud,X. Chen,S. R. McCouch.MGG Molecular & General Genetics . 1996 (5)
  • [7] The mlo resistance alleles to powdery mildew infection in barley trigger a developmentally controlled defence mimic phenotype[J] . Marietta Wolter,Karin Hollricher,Francesco Salamini,Paul Schulze-Lefert.MGG Molecular & General Genetics . 1993 (1)