Effects of follistatin on testosterone secretion of rat Leydig cell in vitro

被引：0
|
作者：
李江源
邵迎红
窦京涛
李明
机构：
[1] Department of Endocrinology
[2] Great Wall Hospital
[3] Bei-jing
来源：
生殖医学杂志 | 2003年 / 11(S1)卷 / S1期
关键词：
Follistatin; Testosterone; Leydig cell;
D O I：
暂无
中图分类号：
R341 [];
学科分类号：
摘要：
<正> Objective:To investigate the effects of follistatin（rhFS-288） on biosynthesis andsecretion of testosterone in rat Leydig cell in vitro.Methods:Leydig cells were isolated from Wistar rat testes by a discontinuous Per-coll gradient procedure.Purified cells were incubated in 24-well plate（10～5 cell/ml/well）and maintained for 24 h in a CO2 incubator,rhFS-288 and Ca2+ were added to the wellsindependently or jointly in both baseline （without hCG） and stimulation condition （1.0IU/ml of hCG） to observe the change of testosterone concentration in the media.Results:rhFS-288 showed a dose-dependent inhibiting effect on testosterone releasein baseline and stimulating condition.Ca2+ presented inhibitory effect either.Whereas,escape phenomenon emerged while Ca2+ concentration reached to 100 mmol/L.A com-bination of rhFS-288 with Ca2+ displayed a dose-dependent inhibition on testosterone se-cretion.Conclusion:rhFS-288 inhibits testosterone secretion in a dose-dependent manner.Calcium is thought to be the second messenger of FS action.The mechanism of escapephenomenon during high dose of Ca2+ along is unknown.
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页码：9 / 12
页数：4
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