抗肾小球基底膜抗体的抗原决定簇的研究

被引：6

作者：

丁焦生

赵明辉

王海燕

机构：

[1] 北京大学第一医院肾内科

来源：

中华医学杂志 | 2001年 / 14期

关键词：

肾小球基底膜; 抗体; 抗原决定簇;

D O I：

暂无

中图分类号：

R692 [肾疾病];

学科分类号：

摘要：

目的　研究抗肾小球基底膜 (GBM)抗体的抗原决定簇及其与临床表现的关系。方法　以抗Ⅳ型胶原α3 链非胶原区 1[α3(Ⅳ )NC1]单抗 (Mab3)和抗α5(Ⅳ )NC1单抗 (Mab5) ,及辣根过氧化物酶标记的亲和层析纯化的抗GBM自身抗体 (APab HRP) ,作为识别GBM上不同抗原决定簇的探针 ,应用竞争性ELISA法测定 33例抗GBM抗体的抗原决定簇 ,同时初步探讨其与临床表现的关系。结果33例患者血清均可抑制Mab3,30 / 33例可抑制APab HRP ,11/ 33例可抑制Mab5。但不同血清对同一探针 ,同一血清对不同探针的抑制率均不平行。单纯抗GBM抗体组 (2 7例 )对APab HRP的平均抑制率为 5 5 %± 18% ,而抗GBM抗体伴抗中性粒细胞胞浆抗体阳性组 (6例 )仅为 32 %± 2 2 % ,两组相比差异有显著意义 (P =0 .0 1)。在有或无肺出血患者之间 ,对各种探针抑制率的差异均无显著意义(P >0 .0 5 )。结论　抗GBM抗体血清均识别位于α3(Ⅳ )NC1上的主要抗原决定簇。但不同患者所识别的抗原决定簇并不完全一致 ,少数血清可识别α5(Ⅳ )NC1。是否合并ANCA ,所识别的抗原决定簇可能存在差异。

引用

页码：34 / 36

页数：3

共 10 条

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