抑制Runx2的表达增强BMP2诱导的干细胞成软骨分化

被引：0

作者：

孙泽绪

赵辰

廖军义

王琦

徐伟

陈诚

黄伟

机构：

[1] 重庆医科大学附属第一医院

来源：

中国生物工程杂志 | 2016年 / 36卷 / 04期

关键词：

Runx2; 骨形态发生蛋白2; C3H10T1/2细胞; 成软骨分化;

D O I：

10.13523/j.cb.20160409

中图分类号：

Q78 [基因工程（遗传工程）]; R329.2 [人体细胞学];

学科分类号：

071007 ; 0836 ; 090102 ; 100101 ;

摘要：

目的:利用腺病毒介导RNA干扰抑制成骨分化关键转录调控因子Runx2(Runt-related transcription factor 2)的表达,研究其对骨形态发生蛋白2(bone morphogenetic protein 2,BMP2)诱导间充质干细胞(mesenchymal stem cells,MSCs)成软骨分化的影响,并初步探讨相关机制。方法:利用腺病毒Ad-GFP、Ad-BMP2、Ad-SiRunx2感染C3H10T1/2细胞;共分4组:GFP组、BMP2组、BMP2+SiRunx2组和SiRunx2组。Alcian blue染色检测各组软骨细胞基质糖胺聚糖分泌;RTPCR检测Runx2、早期成软骨标志物(Col2a1)、蛋白聚糖(Aggrecan)及晚期成软骨标志物(Col10a1)mRNA表达水平;Western blot检测目的蛋白BMP2、Ⅱ型胶原(Col2a1)及X型胶原(Col10a1)的蛋白表达。结果:在BMP2诱导C3H10T1/2细胞成软骨分化过程中,下调Runx2的表达可以增强Col2a1、Aggrecan及软骨细胞外基质糖胺聚糖的合成,抑制Col10a1的合成。结论:下调Runx2表达可以增强BMP2诱导间充质干细胞成软骨分化能力,并抑制软骨细胞的成熟和肥大。

引用

页码：57 / 62

页数：6

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